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从生物墨水到组织：在可打印性和细胞行为的背景下探索壳聚糖-琼脂糖复合材料。

From Bioink to Tissue: Exploring Chitosan-Agarose Composite in the Context of Printability and Cellular Behaviour.

机构信息

Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland.

Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland.

出版信息

Molecules. 2024 Sep 30;29(19):4648. doi: 10.3390/molecules29194648.

DOI:10.3390/molecules29194648
PMID:39407579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11477700/
Abstract

This study presents an innovative method for producing thermosensitive bioink from chitosan hydrogels saturated with carbon dioxide and agarose. It focuses on a detailed characterisation of their physicochemical properties and potential applications in biomedicine and tissue engineering. The ORO test approved the rapid regeneration of the three-dimensional structure of chitosan-agarose composites in a unidirectional bench press simulation test. The diffusion of dyes through the chitosan-agarose hydrogel membranes strongly depended on the share of both polymers in the composite and the molecular weight of the dyes. Glucose, as a nutrient marker, also diffused through all membranes regardless of composition. Biocompatibility assessment using MTT tests on 46BR.1N fibroblasts and HaCaT keratinocytes confirmed the safety of the bioink. The regenerative potential of the bioink was confirmed by efficient cell migration, especially HaCaT. Long-term viability studies showed that chitosan-agarose scaffolds, unlike the agarose ones, support cell proliferation and survival, especially 14 days after bioink extrusion. Experiments in a skin wound model in mice confirmed the biocompatibility of the tested dressing and the beneficial action of chitosan on healing. Studies on vessel formation in chicken embryos highlight the potential of the chitosan-agarose composition to enhance proangiogenic effects. This composition meets all entry criteria and possesses excellent biological properties.

摘要

本研究提出了一种从二氧化碳饱和的壳聚糖水凝胶和琼脂糖中制备温敏生物墨水的创新方法。重点对其物理化学性质及其在生物医学和组织工程中的潜在应用进行了详细表征。ORO 试验证实了壳聚糖-琼脂糖复合材料在单向压模模拟试验中能够快速再生三维结构。染料在壳聚糖-琼脂糖水凝胶膜中的扩散强烈依赖于复合材料中两种聚合物的比例和染料的分子量。葡萄糖作为营养标记物，无论组成如何，都能通过所有膜扩散。通过 MTT 试验对 46BR.1N 成纤维细胞和 HaCaT 角质细胞进行的生物相容性评估证实了生物墨水的安全性。生物墨水的再生潜力通过有效细胞迁移得到证实，特别是 HaCaT。长期生存能力研究表明，壳聚糖-琼脂糖支架与琼脂糖支架不同，支持细胞增殖和存活，尤其是在生物墨水挤出 14 天后。在小鼠皮肤伤口模型中的实验证实了所测试敷料的生物相容性以及壳聚糖对愈合的有益作用。鸡胚血管生成实验突出了壳聚糖-琼脂糖组成物增强促血管生成作用的潜力。该组合物符合所有入选标准，并具有优异的生物学特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/5a695dee9681/molecules-29-04648-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/acbbf495ade3/molecules-29-04648-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/cf25a2cdee71/molecules-29-04648-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/5d446160ce15/molecules-29-04648-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/672ac2e9abe1/molecules-29-04648-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/1ee5b3a30734/molecules-29-04648-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/a543bfef211d/molecules-29-04648-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/26ac35f0faff/molecules-29-04648-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/9d637354669a/molecules-29-04648-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/5a695dee9681/molecules-29-04648-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/acbbf495ade3/molecules-29-04648-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/cf25a2cdee71/molecules-29-04648-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/5d446160ce15/molecules-29-04648-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/672ac2e9abe1/molecules-29-04648-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/1ee5b3a30734/molecules-29-04648-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/a543bfef211d/molecules-29-04648-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/26ac35f0faff/molecules-29-04648-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/9d637354669a/molecules-29-04648-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3563/11477700/5a695dee9681/molecules-29-04648-g009.jpg

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