Alonso M C, Navarrete C, Solana R, Torres A, Pena J, Festenstein H
Tissue Antigens. 1985 Nov;26(5):310-7. doi: 10.1111/j.1399-0039.1985.tb02229.x.
We have previously shown that HLA-class II antigens have a differential expression on acute myeloblastic leukaemia (AML) blasts. These cells express HLA-DR molecules but the HLA-DQ antigens are undetectable. In this paper we study the expression of HLA-DR and HLA-DQ antigens, using monoclonal antibodies (MoAbs), on normal cells of the myelomonocytic lineage: the common myelomonocytic progenitor (CFU-GM) and the monocytes, by techniques of inhibition of CFU-GM growth and double immunofluoroscence, respectively. The results show that HLA-DR and HLA-DQ antigens are differentially expressed on normal myelomonocytic cells. While HLA-DR molecules are expressed on CFU-GM and on the majority of peripheral blood monocytes, HLA-DQ antigens are not expressed on CFU-GM and only present on a subpopulation of monocytes. This data further confirms that HLA-DR and HLA-DQ molecules are coded by different genes with independent regulation of the gene expression not only on myeloid leukaemias but also on normal cells of the myelomonocytic lineage.
我们先前已表明，HLA - II类抗原在急性髓细胞白血病（AML）原始细胞上有差异表达。这些细胞表达HLA - DR分子，但检测不到HLA - DQ抗原。在本文中，我们分别运用抑制CFU - GM生长技术和双重免疫荧光技术，使用单克隆抗体（MoAbs）研究了髓单核细胞系正常细胞（即常见髓单核祖细胞（CFU - GM）和单核细胞）上HLA - DR和HLA - DQ抗原的表达。结果显示，HLA - DR和HLA - DQ抗原在正常髓单核细胞上有差异表达。虽然HLA - DR分子在CFU - GM和大多数外周血单核细胞上表达，但HLA - DQ抗原在CFU - GM上不表达，仅存在于单核细胞亚群中。该数据进一步证实，HLA - DR和HLA - DQ分子由不同基因编码，其基因表达不仅在髓系白血病细胞上，而且在髓单核细胞系正常细胞上都有独立调控。
