Liu Xinyong, Yu Zixuan, Tong Xiaohong, Chang Longxue, Huang Jie, Wang Yifeng, Ying Jiezheng, Li Xingwang, Ni Shen, Zhang Jian
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2024 Apr 18;13(8):1130. doi: 10.3390/plants13081130.
Anther length is the critical floral trait determining hybrid rice seed production and is controlled by many quantitative trait loci (QTL). However, the cloning of genes specifically controlling anther size has yet to be reported. Here, we report the fine mapping of for anther size using backcross inbred lines (BILs) in the genetic background of Huazhan (HZ). Gene chip analysis on the BCF and BCF population identified effective loci on Chr1, Chr5, and Chr8 and two genomic regions on Chr5, named and . was identified in both populations with LOD values of 17.54 and 10.19, which explained 35.73% and 25.1% of the phenotypic variances, respectively. Ultimately was localized to a 73 kb region between HK139 and HK140 on chromosome 5. And we constructed two near-isogenic lines (NILs) for RNA-seq analysis, named NIL-qAL5.2 and NIL-qAL5.2, respectively. The result of the GO enrichment analysis revealed that differential genes were significantly enriched in the carbohydrate metabolic process, extracellular region, and nucleic acid binding transcription, and KEGG enrichment analysis revealed that alpha-linolenic acid metabolism was significantly enriched. Meanwhile, candidate genes of were analyzed in RNA-seq, and it was found that is differentially expressed between NIL-qAL5.2 and NIL-qAL5.2. The fine mapping of conferring anther length will promote the breed improvement of the restorer line and understanding of the mechanisms driving crop mating patterns.
花药长度是决定杂交水稻制种的关键花部性状，受多个数量性状位点（QTL）控制。然而，尚未见专门控制花药大小的基因克隆报道。本文报道了在华占（HZ）遗传背景下利用回交自交系（BILs）对控制花药大小的基因进行精细定位。对BCF和BCF群体进行基因芯片分析，在第1、5和8染色体上鉴定到有效位点，在第5染色体上鉴定到两个基因组区域，分别命名为和。在两个群体中均鉴定到，其LOD值分别为17.54和10.19，分别解释了35.73%和25.1%的表型变异。最终，被定位到第5染色体上HK139和HK140之间的73 kb区域。我们构建了两个用于RNA测序分析的近等基因系（NILs），分别命名为NIL-qAL5.2和NIL-qAL5.2。GO富集分析结果显示，差异基因在碳水化合物代谢过程、细胞外区域和核酸结合转录中显著富集，KEGG富集分析显示α-亚麻酸代谢显著富集。同时，在RNA测序中对的候选基因进行了分析，发现其在NIL-qAL5.2和NIL-qAL5.2之间差异表达。对控制花药长度的基因进行精细定位将促进恢复系的品种改良以及对作物交配模式驱动机制的理解。
