Equine piroplasmosis (EP) is caused by (.) and/or (.) . The aim was to assess the percentage of positive test results for EP in horses in Europe and to identify risk factors for pathogen contact/infection. This study included results from PCR and competitive enzyme-linked immunosorbent assay testing requested by European veterinarians between 2008 and 2021. Binary bivariate logistic regression was used to analyze risk factors. A total of 4060 horses were included. PCR testing was positive in 9.7% (154/1589), serology for in 15.2% (393/2591) and for in 6.8% (175/2578). The odds of positive serology increased by 6.8% (, = 0.008) and 9.5% (, < 0.001) each year. Regionality had a statistically significant impact on PCR (Eastern = 0.047/OR = 1.605; Southern = 0.029/OR = 1.451; Central = 0.007/OR = 0.617) and serological testing for (Southern < 0.001/OR = 2.521; Central < 0.001/OR = 0.537; Northern = 0.003/OR = 0.462), as well as breeds on seroprevalence of (heavy horses: = 0.016/OR = 2.239) and (ponies: = 0.007/OR = 0.340; warmbloods: = 0.025/OR = 1.602). In conclusion, there was a significant geographical impact on the results of PCR and serology, consistent with known vector habitats. The rising numbers of horses tested serologically positive highlights the importance of surveillance.