Leuthner Moritz, Reisbeck Mathias, Helou Michael, Hayden Oliver
Heinz-Nixdorf-Chair of Biomedical Electronics, School of Computation, Information and Technology & Munich Institute of Biomedical Engineering, Technical University of Munich, TranslaTUM, Einsteinstraße 25, 81675 Munich, Germany.
EarlyBio GmbH, Bottroper Weg 2, 13507 Berlin, Germany.
Micromachines (Basel). 2024 Apr 13;15(4):520. doi: 10.3390/mi15040520.
For the treatment of human immunodeficiency virus (HIV)-infected patients, the regular assessment of the immune status is indispensable. The quantification of CD4 T lymphocytes in blood by gold standard optical flow cytometry is not point-of-care testing (POCT) compatible. This incompatibility is due to unavoidable pre-analytics, expensive and bulky optics with limited portability, and complex workflow integration. Here, we propose a non-optical, magnetic flow cytometry (MFC) workflow that offers effortless integration opportunities, including minimal user interaction, integrated sample preparation and up-concentration, and miniaturization. Furthermore, we demonstrate immunomagnetic CD4 T lymphocyte labeling in whole blood with subsequent quantification using sheath-less MFC. Showing linearity over two log scales and being largely unimpaired by hematocrit, evidence is provided for POCT capabilities of HIV patients.
对于人类免疫缺陷病毒（HIV）感染患者的治疗，定期评估免疫状态是必不可少的。采用金标准光流式细胞术对血液中的CD4 T淋巴细胞进行定量分析与即时检验（POCT）不兼容。这种不兼容性是由于不可避免的分析前处理、昂贵且笨重的光学器件（便携性有限）以及复杂的工作流程整合所致。在此，我们提出一种非光学的磁流式细胞术（MFC）工作流程，该流程提供了轻松的整合机会，包括最少的用户交互、集成的样品制备和浓缩以及小型化。此外，我们展示了在全血中进行免疫磁性CD4 T淋巴细胞标记，随后使用无鞘MFC进行定量分析。结果表明在两个对数范围内具有线性，并且在很大程度上不受血细胞比容的影响，这为HIV患者的POCT能力提供了证据。
