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利用在氟化钡上制作的二元轴棱锥扩展红外显微镜的焦深

Extending the Depth of Focus of an Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride.

作者信息

Han Molong, Smith Daniel, Kahro Tauno, Stonytė Dominyka, Kasikov Aarne, Gailevičius Darius, Tiwari Vipin, Ignatius Xavier Agnes Pristy, Gopinath Shivasubramanian, Ng Soon Hock, John Francis Rajeswary Aravind Simon, Tamm Aile, Kukli Kaupo, Bambery Keith, Vongsvivut Jitraporn, Juodkazis Saulius, Anand Vijayakumar

机构信息

Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.

Institute of Physics, University of Tartu, 50411 Tartu, Estonia.

出版信息

Micromachines (Basel). 2024 Apr 17;15(4):537. doi: 10.3390/mi15040537.

DOI:10.3390/mi15040537
PMID:38675348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11052387/
Abstract

Axial resolution is one of the most important characteristics of a microscope. In all microscopes, a high axial resolution is desired in order to discriminate information efficiently along the longitudinal direction. However, when studying thick samples that do not contain laterally overlapping information, a low axial resolution is desirable, as information from multiple planes can be recorded simultaneously from a single camera shot instead of plane-by-plane mechanical refocusing. In this study, we increased the focal depth of an infrared microscope non-invasively by introducing a binary axicon fabricated on a barium fluoride substrate close to the sample. Preliminary results of imaging the thick and sparse silk fibers showed an improved focal depth with a slight decrease in lateral resolution and an increase in background noise.

摘要

轴向分辨率是显微镜最重要的特性之一。在所有显微镜中，都希望有高轴向分辨率以便沿纵向有效区分信息。然而，在研究不包含横向重叠信息的厚样品时，低轴向分辨率是可取的，因为可以从单个相机拍摄中同时记录来自多个平面的信息，而无需逐平面进行机械重新聚焦。在本研究中，我们通过在靠近样品的氟化钡衬底上制作二元轴棱锥，非侵入性地增加了红外显微镜的焦深。对厚且稀疏的丝纤维成像的初步结果显示焦深有所改善，但横向分辨率略有下降，背景噪声增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/bad3f942cfe1/micromachines-15-00537-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/36543658a260/micromachines-15-00537-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/baccb75529ed/micromachines-15-00537-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/82f3c6377d16/micromachines-15-00537-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/c8aa4b66ada7/micromachines-15-00537-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/509f5e1e0e23/micromachines-15-00537-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/25d63697878d/micromachines-15-00537-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/0cd9666b0a35/micromachines-15-00537-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/ba30a3aef7c2/micromachines-15-00537-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/af4009b88d11/micromachines-15-00537-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/ed8fe5d5868e/micromachines-15-00537-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/5143f53ba013/micromachines-15-00537-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/bad3f942cfe1/micromachines-15-00537-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/36543658a260/micromachines-15-00537-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/baccb75529ed/micromachines-15-00537-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/82f3c6377d16/micromachines-15-00537-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/c8aa4b66ada7/micromachines-15-00537-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/509f5e1e0e23/micromachines-15-00537-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/25d63697878d/micromachines-15-00537-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/0cd9666b0a35/micromachines-15-00537-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/ba30a3aef7c2/micromachines-15-00537-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/af4009b88d11/micromachines-15-00537-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/ed8fe5d5868e/micromachines-15-00537-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/5143f53ba013/micromachines-15-00537-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4079/11052387/bad3f942cfe1/micromachines-15-00537-g012.jpg

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