Li Baojian, Yang Yuangui, Song Zhongxing, Tang Zhishu
Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
China Academy of Chinese Medical Sciences, Beijing 100700, China.
Pharmaceuticals (Basel). 2024 Mar 25;17(4):413. doi: 10.3390/ph17040413.
Hu (ZS), as a "medicinal and food-homologous" plant, has been used for a long history. The study was to assess the sedative and hypnotic effects among various parts of ZS. The model, diazepam (DZP), ZS kernel (ZSS), ZS flesh (ZSF), and ZS husk (ZSKS) group occurred subsequent to the successful establishment of the para-chlorophenylalanine induced insomnia model via intraperitoneal injection. The latency and duration of sleep in mice in each group were recorded. The substance basis of various parts of ZS was analyzed by the UPLC-QTOF-MS technique. The results showed that relative to the model group, DZP, ZSS, ZSF, and ZSKS groups demonstrated shortened sleep latency ( < 0.05) and extended sleep duration ( < 0.01). The GABA, 5-HT, and BDNF levels were significantly upregulated in the brain tissues of the mice in the DZP, ZSF, and ZSS groups ( < 0.01). However, the improvement in ZSKS was non-significant. Additionally, the mRNA and protein expression levels of 5-HT1AR, GABAARα1, and BDNF in mice in the DZP, ZSS, and ZSF groups were significantly enhanced ( < 0.01). However, the improvement in the ZSKS group was insignificant ( < 0.05). The examination of the substance composition across different parts revealed that the shared chemical basis contributing to the sedative and hypnotic potency of different parts of ZS may involve the presence of compounds such as () magnoflorine, () betulinic acid, () ceanothic acid, and () alphitolic acid. It provides a basis for further elucidation of the substance basis responsible for the functional and medicinal effects of ZS.
胡（ZS）作为一种“药食同源”植物，已有悠久的使用历史。本研究旨在评估ZS各部位的镇静催眠作用。通过腹腔注射成功建立对氯苯丙氨酸诱导的失眠模型后，设立地西泮（DZP）、ZS仁（ZSS）、ZS果肉（ZSF）和ZS壳（ZSKS）组。记录每组小鼠的睡眠潜伏期和睡眠时间。采用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS）技术分析ZS各部位的物质基础。结果显示，与模型组相比，DZP、ZSS、ZSF和ZSKS组的睡眠潜伏期缩短（<0.05），睡眠时间延长（<0.01）。DZP、ZSF和ZSS组小鼠脑组织中的γ-氨基丁酸（GABA）、5-羟色胺（5-HT）和脑源性神经营养因子（BDNF）水平显著上调（<0.01）。然而，ZSKS组的改善不显著。此外，DZP、ZSS和ZSF组小鼠中5-HT1AR、GABAARα1和BDNF的mRNA和蛋白表达水平显著增强（<0.01）。然而，ZSKS组的改善不显著（<0.05）。对不同部位物质组成的检测表明，ZS不同部位镇静催眠作用的共同化学基础可能涉及（）木兰碱、（）桦木酸、（）ceanothic酸和（）齐墩果酸等化合物的存在。这为进一步阐明ZS功能和药用作用的物质基础提供了依据。
