Li Zheng, Zhang Yiqian, Cao Jinping, Xing Xupeng, Liang Yalin, Zhang Yuxing, Tang Xiaopeng, Lin Shengyi, Wu Zhenfang, Li Zicong, Huang Sixiu
National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory of Swine and Poultry Breeding Industry, South China Agricultural University, Guangzhou 510642, China.
Pharmaceuticals (Basel). 2024 Apr 2;17(4):455. doi: 10.3390/ph17040455.
In vitro oocyte maturation (IVM) technology is important for assisted animal and human reproduction. However, the maturation rates and developmental potential of in vitro-matured oocytes are usually lower than those of in vivo-matured oocytes. Oxidative stress is a main factor that causes the lower maturation rates and quality of in vitro-matured oocytes. The purpose of this study was to investigate the effects of treatment with SkQ1, a mitochondria-targeted antioxidant, on mouse IVM and subsequent embryonic development. The results demonstrated that the supplementation of SkQ1 during IVM improves the maturation rates of mouse oocytes and the subsequent developmental competence of in vitro-fertilized embryos. The addition of SkQ1 to the IVM medium also decreased oxidative stress and apoptosis, and increased mitochondrial membrane potential in matured mouse oocytes. This study provides a new method through which to enhance the maturation rates and the quality of in vitro-matured mouse oocytes, thus promoting the application and development of assisted animal and human reproductive technology.
体外卵母细胞成熟（IVM）技术对于辅助动物和人类繁殖至关重要。然而，体外成熟卵母细胞的成熟率和发育潜力通常低于体内成熟的卵母细胞。氧化应激是导致体外成熟卵母细胞成熟率和质量较低的主要因素。本研究的目的是探讨线粒体靶向抗氧化剂SkQ1处理对小鼠IVM及后续胚胎发育的影响。结果表明，在IVM过程中添加SkQ1可提高小鼠卵母细胞的成熟率以及体外受精胚胎的后续发育能力。向IVM培养基中添加SkQ1还可降低氧化应激和细胞凋亡，并增加成熟小鼠卵母细胞的线粒体膜电位。本研究提供了一种新方法，可提高体外成熟小鼠卵母细胞的成熟率和质量，从而促进辅助动物和人类生殖技术的应用和发展。
