Chniguir Amina, Saguem Mohamed Hedi, Dang Pham My-Chan, El-Benna Jamel, Bachoual Rafik
Faculty of Sciences of Gabes, University of Gabes, Gabes 6029, Tunisia.
Laboratory of Anatomy and Pathological Cytology, Gabes 6029, Tunisia.
Pharmaceuticals (Basel). 2024 Apr 15;17(4):504. doi: 10.3390/ph17040504.
Eugenol (Eug) is a polyphenol extracted from the essential oil of (L.) Merr. and Perry (Myrtaceae). The health benefits of eugenol in human diseases were proved in several studies. This work aims to evaluate the effect of eugenol on lung inflammatory disorders. For this, using human neutrophils, the antioxidant activity of eugenol was investigated in vitro. Furthermore, a model of LPS-induced lung injury in mice was used to study the anti-inflammatory effect of eugenol in vivo. Results showed that eugenol inhibits luminol-amplified chemiluminescence of resting neutrophils and after stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLF) peptide or phorbol myristate acetate (PMA). This effect was dose dependent and was significant from a low concentration of 0.1 µg/mL. Furthermore, eugenol inhibited myeloperoxidase (MPO) activity without affecting its degranulation. Eugenol has no scavenging effect on hydrogen peroxide (HO) and superoxide anion (O). Pretreatment of mice with eugenol prior to the administration of intra-tracheal LPS significantly reduced neutrophil accumulation in the bronchoalveolar lavage fluid (BALF) and decreased total proteins concentration. Moreover, eugenol clearly inhibited the activity of matrix metalloproteinases MMP-2 (21%) and MMP-9 (28%), stimulated by LPS administration. These results suggest that the anti-inflammatory effect of eugenol against the LPS-induced lung inflammation could be exerted via inhibiting myeloperoxidase and metalloproteinases activity. Thus, eugenol could be a promising molecule for the treatment of lung inflammatory diseases.
丁香酚（Eug）是从桃金娘科植物丁香（Syzygium aromaticum (L.) Merr. and Perry）的精油中提取的一种多酚。多项研究证实了丁香酚对人类疾病的健康益处。这项工作旨在评估丁香酚对肺部炎症性疾病的影响。为此，使用人中性粒细胞在体外研究了丁香酚的抗氧化活性。此外，还利用脂多糖（LPS）诱导的小鼠肺损伤模型在体内研究了丁香酚的抗炎作用。结果表明，丁香酚可抑制静息中性粒细胞以及在用N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLF）肽或佛波酯（PMA）刺激后的鲁米诺增强化学发光。这种作用呈剂量依赖性，从低浓度0.1μg/mL起就具有显著性。此外，丁香酚抑制髓过氧化物酶（MPO）活性，但不影响其脱颗粒。丁香酚对过氧化氢（HO）和超氧阴离子（O）没有清除作用。在用气管内注射LPS之前用丁香酚预处理小鼠，可显著减少支气管肺泡灌洗液（BALF）中的中性粒细胞积聚，并降低总蛋白浓度。此外，丁香酚明显抑制了LPS给药刺激的基质金属蛋白酶MMP-2（21%）和MMP-9（28%）的活性。这些结果表明，丁香酚对LPS诱导的肺部炎症的抗炎作用可能是通过抑制髓过氧化物酶和金属蛋白酶的活性来实现的。因此，丁香酚可能是治疗肺部炎症性疾病的一种有前景的分子。
