de Oliveira Laís Albuquerque, de Morais Isa Rita Brito, Barbosa Marcelo Dos Santos, Beutinger Marchioro Silvana, Leite Machado Layla Oliveira Campos, Ferreira Marques Michele, da Silva Ferreira Tiago, de Almeida Gabriel Barroso, de Oliveira Barbosa Dyjaene, Leite Torres Alex José, Simionatto Simone
Health Science Research Laboratory, Federal University of Grande Dourados, Dourados 79804-970, MS, Brazil.
Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia, Salvador 40170-110, BA, Brazil.
Vaccines (Basel). 2024 Apr 10;12(4):402. doi: 10.3390/vaccines12040402.
Although the adaptive immune responses to the CoronaVac vaccine are known, their dynamics in indigenous communities remain unclear. In this study, we assessed the humoral and cellular immune responses to CoronaVac (Sinovac Biotech Life Sciences, 2021 NCT05225285, Beijing, China), in immunized Brazilian indigenous individuals.
We conducted a prospective cohort study on indigenous Brazilian people between February 2021 and June 2021. Analyses of immune responses were carried out before (T1) and after a vaccination schedule was completed (T2). Demographic data were collected using a questionnaire.
We initially included 328 patients; among them, 120 (36.6%) had no SARS-CoV-2 antibodies. Peripheral blood mononuclear cells (PBMCs) were collected from 106 patients during follow-up visits, of which 91 samples were analyzed by immunophenotyping assay to detect SARS-CoV-2-specific memory T-cell response. Post-vaccination, the levels of memory B-cells and Natural Killer T-lymphocytes increased. Bororó village residents, females, and Terena ethnic group members had higher levels of anti-spike IgG antibodies post-vaccination, whereas alcohol and tobacco users had lower concentrations.
To our best knowledge, this was the first comprehensive assessment of antibody and T-cell responses against CoronaVac vaccination in indigenous patients. Our findings showed that antibody response and T-cell immunity against SARS-CoV-2 were present in most patients following the vaccination schedule.
虽然对科兴新冠疫苗的适应性免疫反应已为人所知，但其在原住民社区中的动态变化仍不清楚。在本研究中，我们评估了巴西原住民个体接种科兴新冠疫苗（北京科兴中维生物技术有限公司，2021，NCT05225285）后的体液免疫和细胞免疫反应。
我们于2021年2月至2021年6月对巴西原住民进行了一项前瞻性队列研究。在完成疫苗接种计划之前（T1）和之后（T2）进行免疫反应分析。使用问卷收集人口统计学数据。
我们最初纳入了328名患者；其中，120名（36.6%）没有新冠病毒抗体。在随访期间从106名患者中采集了外周血单个核细胞（PBMC），其中91份样本通过免疫表型分析检测新冠病毒特异性记忆T细胞反应。接种疫苗后，记忆B细胞和自然杀伤T淋巴细胞水平升高。博罗罗村居民、女性和特雷诺族成员接种疫苗后抗刺突IgG抗体水平较高，而酗酒和吸烟人群的抗体浓度较低。
据我们所知，这是首次对原住民患者接种科兴新冠疫苗后的抗体和T细胞反应进行的全面评估。我们的研究结果表明，在完成疫苗接种计划后，大多数患者体内存在针对新冠病毒的抗体反应和T细胞免疫。
