Safety Assessment of UBSS-01 in Rats and Double-Blind Placebo-Controlled Study in Healthy Individuals.

is a common, harmless, and prevalent member of the oral microbiota in humans. In the present study, the safety of UBSS-01 was evaluated using in silico methods and preclinical and clinical studies. In an acute toxicity study, rats were administered with 5 g/kg (500 × 10 CFU) UBSS-01. The changes in phenotypic behaviors and hematological, biochemical, electrolytes, and urine analyses were monitored. No toxicity was observed at 14 days post-treatment. The no observable effects limit (NOEL) of UBSS-01 was >5 g/kg in rats. In a 28-day repeat dose toxicity study, rats were administered UBSS-01 once daily at doses of 0.1, 0.5, and 1 g/kg (10, 50, and 100 billion CFU/kg, respectively) body weight. UBSS-01 did not influence any of the hematology parameters and clinical chemistry parameters in plasma and serum samples after 28-day repeated administration. No structural abnormality was observed in the histological examination of organs. Whole genome analysis revealed the absence of virulence factors or genes that may transmit antibiotic resistance. In the double-blind study with 60 human participants (aged 18-60 years), consumption of UBSS-01 for 30 days was found to be safe and results were comparable with placebo treatment These findings indicate that UBSS-01 may be safe for human consumption.