Department of Biosciences, Galgotias University, Greater Noida, Uttar Pradesh, India.
Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Med Oncol. 2024 Apr 27;41(6):130. doi: 10.1007/s12032-024-02374-w.
The Fucaceae family of marine brown algae includes Ascophyllum nodosum. Fucosterol (FSL) is a unique bioactive component that was identified through GC-MS analysis of the hydroalcoholic extract of A. nodosum. Fucosterol's mechanism of action towards hepatocellular cancer was clarified using network pharmacology and docking study techniques. The probable target gene of FSL has been predicted using the TargetNet and SwissTargetPred databases. GeneCards and the DisGNet database were used to check the targeted genes of FSL. By using the web programme Venny 2.1, the overlaps of FSL and HCC disease demonstrated that 18 genes (1.3%) were obtained as targeted genes Via the STRING database, a protein-protein interaction (PPI) network with 18 common target genes was constructed. With the aid of CytoNCA, hub genes were screened using the Cytoscape software, and the targets' hub genes were exported into the ShinyGo online tool for study of KEGG and gene ontology enrichment. Using the software AutoDock, a hub gene molecular docking study was performed. Ten genes, including AR, CYP19A1, ESR1, ESR2, TNF, PPARA, PPARG, HMGCR, SRC, and IGF1R, were obtained. The 10 targeted hubs docked with FSL successfully. The active components FSL of ASD, the FSL, are engaged in fatty liver disease, cancer pathways, and other signalling pathways, which could prove beneficial for the management of HCC.
马尾藻科的海洋褐藻包括裙带菜。岩藻甾醇（FSL）是一种独特的生物活性成分，通过 GC-MS 分析裙带菜的水醇提取物中鉴定得到。通过网络药理学和对接研究技术阐明了岩藻甾醇对肝癌细胞的作用机制。使用 TargetNet 和 SwissTargetPred 数据库预测了 FSL 的可能靶基因。使用 GeneCards 和 DisGNet 数据库检查 FSL 的靶向基因。通过使用网络程序 Venny 2.1，FSL 和 HCC 疾病的重叠表明，通过 STRING 数据库，构建了一个包含 18 个共同靶基因的蛋白质-蛋白质相互作用（PPI）网络。借助 CytoNCA，使用 Cytoscape 软件筛选枢纽基因，并将目标基因的枢纽基因导出到 ShinyGo 在线工具中进行 KEGG 和基因本体富集研究。使用软件 AutoDock 进行了枢纽基因分子对接研究。获得了包括 AR、CYP19A1、ESR1、ESR2、TNF、PPARA、PPARG、HMGCR、SRC 和 IGF1R 在内的 10 个基因。10 个靶基因与 FSL 成功对接。ASD 的活性成分 FSL 参与了脂肪肝疾病、癌症通路和其他信号通路，这可能有助于 HCC 的治疗。
