International Graduate Program of Medicines, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Department of Pharmacology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Methods Mol Biol. 2024;2803:123-136. doi: 10.1007/978-1-0716-3846-0_9.
Isoproterenol (ISO) administration produces significant biochemical and histological changes including oxidative stress, reactive oxygen species (ROS) overproduction, and inflammation that leads to aggravation of myocardial injury. Subcutaneous or intraperitoneal ISO injection into rats can replicate several features of human heart disease, making it a useful tool for comprehending the underlying mechanisms and evaluating potential therapeutic strategies. In the present chapter, we elaborate on how depending on the precise experimental goals and the intended level of severity, different dosages and regimens are employed to induce myocardial injury.
异丙肾上腺素（ISO）的使用会导致显著的生化和组织学变化，包括氧化应激、活性氧（ROS）过度产生和炎症，从而加重心肌损伤。将 ISO 通过皮下或腹腔注射到大鼠体内，可以复制出人类心脏病的多种特征，因此它是一种有用的工具，可以帮助我们理解潜在的发病机制并评估潜在的治疗策略。在本章中，我们将详细说明，根据具体的实验目标和预期的严重程度，如何使用不同的剂量和方案来诱导心肌损伤。
