Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran; Department of Physiology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Life Sci. 2019 Jun 15;227:187-192. doi: 10.1016/j.lfs.2019.04.056. Epub 2019 Apr 24.
Myocardial injury (MI) is the principal cause of death from cardiovascular disease (CVD). The present study was conducted to investigate the ameliorative and antioxidant effects of cerebrolysin (CBL) on isoproterenol-induced MI in rats.
MI was induced in the rats by subcutaneously injecting 100 mg/kg of isoproterenol (ISO) in the first two days. The serum levels of creatine phosphokinase (CK-MB) and cardiac troponin I (cTnI) were measured on the third day to confirm MI. The post-treatment involved intraperitoneally injecting 5 ml/kg of CBL for 7 days. Nitric oxide (NO), malondialdehyde (MDA) in the heart tissue and catalase (CAT) and serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were measured on the 10th day using the enzyme-linked immunosorbent assay (ELISA). Histopathological examinations of the heart tissue were also performed.
The present results suggested significant increases in CK-MB, cTnI, MDA and NO. A significant decrease was also observed in the ISO-treated rats in certain antioxidant enzymes, including CAT and GPX. CBL administration showed a significant ameliorative increase against the oxidative ISO-induced damage. Moreover, the histopathological findings showed lower levels of the infiltration of inflammatory cells and edema and vascular proliferation in the CBL-treated rats.
The present histopathological and biochemical findings attributed antioxidant properties to CBL in the rat myocardium and suggested protective effects on ISO-induced MI.
心肌损伤（MI）是心血管疾病（CVD）死亡的主要原因。本研究旨在探讨脑活素（CBL）对异丙肾上腺素诱导的大鼠 MI 的改善和抗氧化作用。
通过在头两天皮下注射 100mg/kg 的异丙肾上腺素（ISO）诱导大鼠 MI。第三天测量血清肌酸磷酸激酶（CK-MB）和心肌肌钙蛋白 I（cTnI）的水平以确认 MI。随后，大鼠腹腔内注射 5ml/kg 的 CBL 进行 7 天的治疗。第 10 天，使用酶联免疫吸附试验（ELISA）测量心脏组织中的一氧化氮（NO）、丙二醛（MDA）和过氧化氢酶（CAT）以及血清中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）的水平。还对心脏组织进行了组织病理学检查。
本研究结果表明，CK-MB、cTnI、MDA 和 NO 显著增加。ISO 处理的大鼠中的某些抗氧化酶，包括 CAT 和 GPX，也显著减少。CBL 给药显示出对氧化 ISO 诱导损伤的显著改善作用。此外，组织病理学发现 CBL 治疗的大鼠中炎性细胞浸润、水肿和血管增生的程度较低。
本研究的组织病理学和生化结果归因于 CBL 在大鼠心肌中的抗氧化特性，并提示其对 ISO 诱导的 MI 具有保护作用。
