硫酸亚铁联合超声乳化肉桂醛纳米乳诱导大肠杆菌 O157:H7 发生铁死亡。
Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7.
机构信息
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
出版信息
Ultrason Sonochem. 2024 Jun;106:106884. doi: 10.1016/j.ultsonch.2024.106884. Epub 2024 Apr 20.
The purpose of this study was to investigate ferroptosis in Escherichia coli O157:H7 caused by ferrous sulfate (FeSO) and to examine the synergistic effectiveness of FeSO combined with ultrasound-emulsified cinnamaldehyde nanoemulsion (CALNO) on inactivation of E. coli O157:H7 in vitro and in vivo. The results showed that FeSO could cause ferroptosis in E. coli O157:H7 via generating reactive oxygen species (ROS) and exacerbating lipid peroxidation. In addition, the results indicated that FeSO combined with CALNO had synergistic bactericidal effect against E. coli O157:H7 and the combined treatment could lead considerable nucleic acids and protein to release by damaging the cell membrane of E. coli O157:H7. Besides, FeSO combined with CALNO had a strong antibiofilm ability to inhibit E. coli O157:H7 biofilm formation by reducing the expression of genes related on biofilm formation. Finally, FeSO combined with CALNO exhibited the significant antibacterial activity against E. coli O157:H7 in hami melon and cherry tomato.
本研究旨在探究硫酸亚铁（FeSO）引起大肠杆菌 O157:H7 发生铁死亡的情况，并考察 FeSO 与超声乳化肉桂醛纳米乳（CALNO）联合应用对体外和体内大肠杆菌 O157:H7 灭活的协同效果。结果表明，FeSO 通过产生活性氧（ROS）和加剧脂质过氧化，导致大肠杆菌 O157:H7 发生铁死亡。此外，结果表明，FeSO 与 CALNO 联合应用对大肠杆菌 O157:H7 具有协同杀菌作用，且联合处理可通过破坏大肠杆菌 O157:H7 的细胞膜，导致大量核酸和蛋白质释放。此外，FeSO 与 CALNO 联合应用具有很强的抗生物膜能力，通过降低生物膜形成相关基因的表达，抑制大肠杆菌 O157:H7 生物膜形成。最后，FeSO 与 CALNO 联合应用对哈密瓜和樱桃番茄中的大肠杆菌 O157:H7 表现出显著的抗菌活性。
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