College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China.
College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan, China; Key Laboratory of Development, Utilization, Quality and Safety Control of Characteristic Agricultural Resources in Central Hunan Province, Loudi, 417000, Hunan, China.
Environ Res. 2024 Jul 1;252(Pt 3):119007. doi: 10.1016/j.envres.2024.119007. Epub 2024 Apr 25.
Global warming and environmental pollutants both pose a threat to the behavior and physiology of animals, but research on the combined effects of the two is limited. Atrazine, a widely used herbicide, has toxic effects on organisms. In this study, the effects of environmental concentrations of atrazine exposure (100 μg/L) for seven days on the movement, metabolism and gene expression related to motility of Pelophylax nigromaculatus larvae (GS8) were investigated under global warming. The results showed that compared to the optimal growth temperature (18 °C), atrazine treatment under global warming (21 °C) significantly increased the average speed (about 11.2 times) and maximum acceleration (about 1.98 times) of P. nigromaculatus larvae, altered the relative abundance of 539 metabolites, including Formyl-5-hydroxykynurenamine, 2,4-Dihydroxybenzophenone, and FAPy-adenine, and changed the nucleotide metabolism, pyrimidine metabolism, glycerophospholipid metabolism, and purine metabolism, as well as increased the gene expression of SPLA2 (about 6.46 times) and CHK (about 3.25 times). In summary, atrazine treatment under global warming caused metabolic disorders in amphibian larvae and increased the expression of some movement-related genes in the brain, resulting in abnormally active.
全球变暖与环境污染物均对动物行为和生理产生威胁，但二者联合效应的研究较为有限。阿特拉津作为一种广泛使用的除草剂，对生物具有毒性作用。本研究探讨了全球变暖条件下，暴露于环境浓度阿特拉津（100μg/L）7 天对黑斑蛙（GS8）幼虫运动、代谢及与运动相关基因表达的影响。结果表明，与最适生长温度（18°C）相比，全球变暖条件下（21°C）阿特拉津处理显著增加了黑斑蛙幼虫的平均速度（约 11.2 倍）和最大加速度（约 1.98 倍），改变了 539 种代谢物的相对丰度，包括 Formyl-5-hydroxykynurenamine、2,4-Dihydroxybenzophenone 和 FAPy-adenine，并改变了核苷酸代谢、嘧啶代谢、甘油磷脂代谢和嘌呤代谢，同时增加了 SPLA2（约 6.46 倍）和 CHK（约 3.25 倍）的基因表达。综上，全球变暖条件下阿特拉津处理导致了两栖类幼虫的代谢紊乱，并增加了脑中某些与运动相关基因的表达，导致其行为异常活跃。
