Department of Thyroid and Breast, The Second Hospital of Nanjing, Nanjing, China.
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):90-94. doi: 10.14715/cmb/2024.70.4.14.
Breast cancer (BC) is one of the most common fatal cancers. Recent studies have identified the vital role of long noncoding RNA (lncRNAs) in the development and progression of BC. In this research, lncRNA PCAT-1 was studied to identify how it functioned in the metastasis of BC. PCAT-1 expression of tissues was detected by real-time quantitative polymerase chain reaction (RT-qPCR) in 50 BC patients. Cell proliferation, wound healing assay and transwell assay were used to observe the biological behavior changes of BC cells through knockdown or overexpression of PCAT-1. In addition, RT-qPCR and Western blot assay were performed to discover the potential target protein of PCAT-1 in BC. PCAT-1 expression level in BC samples was higher than that of adjacent ones. Besides, cell proliferation, migrated ability and cell invaded ability of BC cells were inhibited after PCAT-1 was silenced. Cell proliferation, migration and invasion of BC cells were promoted after PCAT-1 was overexpressed. In addition, SOX4 was downregulated after silence of PCAT-1 in BC cells, while SOX4 was upregulated after overexpression of PCAT-1 in BC cells. Furthermore, SOX4 was upregulated in BC tissues and was positively associated with PCAT-1. Our study uncovers a new oncogene in BC and suggests that PCAT-1 could enhance BC cell proliferation, migration and invasion via targeting SOX4, which provided a novel therapeutic target for BC patients.
乳腺癌（BC）是最常见的致命癌症之一。最近的研究已经确定了长非编码 RNA（lncRNA）在 BC 发展和进展中的重要作用。在这项研究中，研究了 lncRNA PCAT-1，以确定其在 BC 转移中的作用方式。通过实时定量聚合酶链反应（RT-qPCR）检测 50 例 BC 患者组织中的 PCAT-1 表达。通过敲低或过表达 PCAT-1，通过细胞增殖、划痕愈合试验和 Transwell 试验观察 BC 细胞生物学行为的变化。此外，还进行了 RT-qPCR 和 Western blot 试验，以发现 PCAT-1 在 BC 中的潜在靶蛋白。BC 样本中的 PCAT-1 表达水平高于相邻样本。此外，沉默 PCAT-1 后 BC 细胞的增殖、迁移和侵袭能力受到抑制。过表达 PCAT-1 后，BC 细胞的增殖、迁移和侵袭能力增强。此外，沉默 PCAT-1 后 BC 细胞中的 SOX4 下调，而过表达 PCAT-1 后 BC 细胞中的 SOX4 上调。此外，SOX4 在 BC 组织中上调，并与 PCAT-1 呈正相关。我们的研究揭示了 BC 中的一个新癌基因，并表明 PCAT-1 可以通过靶向 SOX4 增强 BC 细胞的增殖、迁移和侵袭，为 BC 患者提供了一个新的治疗靶点。
