lncRNA BG981369 通过 SOX4 信号通路抑制人胃癌细胞增殖、迁移和侵袭并促进细胞凋亡。
lncRNA BG981369 Inhibits Cell Proliferation, Migration, and Invasion, and Promotes Cell Apoptosis by SRY-Related High-Mobility Group Box 4 (SOX4) Signaling Pathway in Human Gastric Cancer.
机构信息
Department of Gastroenterology and Hepatology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Department of Pediatrics, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China (mainland).
出版信息
Med Sci Monit. 2018 Feb 5;24:718-726. doi: 10.12659/msm.905965.
BACKGROUND Human gastric cancer (GC) is a leading primary cause of cancer-associated deaths in both males and females worldwide. However, there are few effective diagnostic and therapeutic measures for GC patients due to the complicated underlying mechanisms of GC. Recently, increasing research has indicated that lncRNAs may play a critical role in the progression of GC. MATERIAL AND METHODS AI769947, AK054978, DB077273, BG981369, AK054588, and AF131784 expressions were analyzed by qRT-PCR assay in GC tissues and corresponding normal tissues (n=44). BG981369 expression was detected by qRT-PCR assay in GC cells. BG981369 was overexpressed and silenced in AGS and SNU-5 cells. The proliferation ability was detected by MTT and colony formation assays. Cell cycle distribution and cell apoptosis rate were analyzed by flow cytometry. The migration and invasion abilities were measured by Transwell assay. In addition, SOX4 expression was analyzed by qRT-PCR in GC tissues. The correlation between SOX4 and BG981369 was analyzed by Pearson analysis. RESULTS The results indicated that lncRNA BG981369 was significantly higher in GC tissues than in normal tissues. Overexpression of BG981369 inhibited the proliferation, migration, and invasion and promoted apoptosis of gastric adenocarcinoma (AGS) cells, and silencing of BG981369 promoted proliferation, migration, and invasion, and inhibited cell apoptosis of SNU-5 cells. Furthermore, we found that SOX4 may act as a downstream mediator of BG981369, suggesting that BG981369 inhibits proliferation, migration, and invasion, and promotes apoptosis by targeting SOX4 in the GC cell lines. CONCLUSIONS Our results suggest that BG981369 and SOX4 are potentially effective therapeutic targets for GC.
背景
人类胃癌（GC）是全球男性和女性癌症相关死亡的主要原因。然而，由于 GC 的复杂潜在机制，GC 患者几乎没有有效的诊断和治疗措施。最近，越来越多的研究表明，lncRNAs 可能在 GC 的进展中发挥关键作用。
方法
通过 qRT-PCR 检测 44 例 GC 组织和相应正常组织中 AI769947、AK054978、DB077273、BG981369、AK054588 和 AF131784 的表达。通过 qRT-PCR 检测 GC 细胞中 BG981369 的表达。在 AGS 和 SNU-5 细胞中过表达和沉默 BG981369。通过 MTT 和集落形成实验检测增殖能力。通过流式细胞术分析细胞周期分布和细胞凋亡率。通过 Transwell 测定法测量迁移和侵袭能力。此外，通过 qRT-PCR 分析 GC 组织中的 SOX4 表达。通过 Pearson 分析分析 SOX4 和 BG981369 之间的相关性。
结果
结果表明，lncRNA BG981369 在 GC 组织中明显高于正常组织。BG981369 的过表达抑制胃腺癌（AGS）细胞的增殖、迁移和侵袭，促进细胞凋亡，而 BG981369 的沉默促进 SNU-5 细胞的增殖、迁移和侵袭，抑制细胞凋亡。此外，我们发现 SOX4 可能是 BG981369 的下游介质，表明 BG981369 通过靶向 GC 细胞系中的 SOX4 抑制增殖、迁移和侵袭，促进凋亡。
结论
我们的结果表明，BG981369 和 SOX4 可能是 GC 的潜在有效治疗靶点。
Zotero 插件