Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon, India.
Department of Transfusion Medicine Medanta-The Medicity, Sector-38, Gurgaon, India.
Transfus Apher Sci. 2024 Jun;63(3):103937. doi: 10.1016/j.transci.2024.103937. Epub 2024 Apr 24.
For assessment of COVID-19 vaccine efficacy, neutralization activity of anti-SARS-CoV-2 antibody is measured. This study was undertaken to determine optimum levels of binding antibody units (BAU/ml) in new quantitative chemiluminescent assay (CLIA) that corresponded to neutralizing potential (30% inhibition) of sVNT assay.
Ninety-one blood samples were analyzed by CLIA and sVNT assays. Test samples (n = 75) were collected from blood donors post-2nd vaccination dose, while control samples (n = 16) were archived pre-COVID donor samples. Correlation between CLIA and sVNT was calculated and receiver operating characteristic (ROC) curve was drawn and analyzed.
Results indicated excellent correlation between 57.5 BAU/ml on CLIA and 30%inhibition on sVNT assay. ROC curve analysis revealed that the area under the curve (AUC) was 0.971.
The present study determined that 57.5 BAU/ml on CLIA corresponded to 30% inhibition on sVNT assay. Periodic quantitative analysis.
为评估 COVID-19 疫苗的功效，会测量抗 SARS-CoV-2 抗体的中和活性。本研究旨在确定新的定量化学发光测定法（CLIA）中与 sVNT 测定的中和潜力（30%抑制率）相对应的最佳结合抗体单位（BAU/ml）水平。
通过 CLIA 和 sVNT 检测分析 91 份血样。检测样本（n=75）取自接种第二剂疫苗后的献血者，而对照样本（n=16）为存档的 COVID 前供体样本。计算 CLIA 和 sVNT 之间的相关性，并绘制和分析接收者操作特性（ROC）曲线。
结果表明，CLIA 上的 57.5 BAU/ml 与 sVNT 检测上的 30%抑制率之间具有极好的相关性。ROC 曲线分析显示曲线下面积（AUC）为 0.971。
本研究确定 CLIA 上的 57.5 BAU/ml 相当于 sVNT 检测上的 30%抑制率。定期进行定量分析。
