College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China.
College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China.
Int J Biol Macromol. 2024 Jun;270(Pt 1):131917. doi: 10.1016/j.ijbiomac.2024.131917. Epub 2024 Apr 26.
Enzymatic degradation of alginate for the preparation of alginate oligosaccharides (AOS) is currently receiving significant attention in the field. AOS has been shown to promote crop growth and improve plant resistance to abiotic stresses. In this study, two PL6 family alginate lyases, AlyRmA and AlyRmB, were expressed and characterized. These enzymes demonstrate exceptional activity and stable thermophilicity compared to other known alginate lyases. AlyRmA (8855.34 U/mg) and AlyRmB (7879.44 U/mg) exhibited excellent degradation activity towards sodium alginate even at high temperatures (70 °C). The AlyRmA and AlyRmB were characterized and utilized to efficiently produce AOS. The study investigated the promotional effect of AOS on the growth of Brassica napus L. seedlings in a saline-alkaline environment. The results of this study demonstrate the high activity and thermal stability of AlyRmA and AlyRmB, highlighting their potential in the preparation of AOS. Moreover, the application of AOS prepared by AlyRmB could enhance the resistance of Brassica napus L. to saline-alkali environments, thereby broadening the potential applications of AOS.
目前，在该领域中，人们对用于制备藻酸盐寡糖（AOS）的酶促降解方法非常关注。研究表明，AOS 可以促进作物生长并提高植物对非生物胁迫的抗性。本研究中，表达并表征了两种 PL6 家族的藻酸盐裂解酶 AlyRmA 和 AlyRmB。与其他已知的藻酸盐裂解酶相比，这些酶具有出色的活性和稳定的耐热性。AlyRmA（8855.34 U/mg）和 AlyRmB（7879.44 U/mg）在高温（70°C）下对海藻酸钠也表现出极好的降解活性。对 AlyRmA 和 AlyRmB 进行了表征，并利用其高效地生产 AOS。该研究调查了 AOS 在盐碱性环境中对油菜幼苗生长的促进作用。本研究结果表明，AlyRmA 和 AlyRmB 具有高活性和热稳定性，这突出了它们在 AOS 制备中的应用潜力。此外，AlyRmB 制备的 AOS 的应用可以增强油菜对盐碱性环境的抗性，从而拓宽了 AOS 的潜在应用。
