Department of Radiologic Technology, Faculty of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Med Sci. 2024 Apr 1;49(4):237-246. doi: 10.30476/ijms.2023.97695.2952. eCollection 2024 Apr.
Cell phone and Ultra-High Frequency (UHF) waves produce oxidative stress and cause testicular toxicity. This investigation was directed to evaluate the effectiveness of Rosmarinic Acid (RA) against oxidative stress caused by UHF radiation in rats.
Forty-two male Wistar rats were divided into six groups. The control received 5 mL normal saline (0.9% NaCl) by gavage, the cell phone group received 915 MHz, the UHF waves group just received 2450 MHz, the RA/cell phone group received RA plus 915 MHz, RA/UHF waves group received RA plus 2450 MHz, and RA just received RA (20 mg/kg). After 30 days of consecutive radiation, the biochemical and histopathological parameters of their testes were measured. Statistical comparison was made using one-way ANOVA followed by Tukey's test.
Cell phone and UHF wave radiation significantly diminished the activity of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase, and glutathione content (P<0.001). On the opposite, UHF significantly increased oxidative stress indices including malondialdehyde level, nitric oxide level, and protein carbonyl content (P<0.001). UHF also significantly reduced the number of Sertoli cells, spermatogonia, primary spermatocyte, epithelial height, and seminiferous tubular and luminal diameters (P<0.001). RA, as an effective antioxidant, reverses the above-mentioned harms and moderates the adverse effects of UHF on the testes of rats by significantly diminishing the oxidative stress indices and antioxidant enzyme rise and improving the histological parameters (P<0.001).
RA can protect the testes of rats from UHF-induced toxicity by reducing oxidative stress. RA as a food supplement might be useful for protecting humans exposed to UHF environmental contamination.
手机和超高频（UHF）波会产生氧化应激，导致睾丸毒性。本研究旨在评估迷迭香酸（RA）对 UHF 辐射引起的大鼠氧化应激的有效性。
将 42 只雄性 Wistar 大鼠分为六组。对照组灌胃给予 5 mL 生理盐水（0.9% NaCl），手机组接受 915 MHz，UHF 波组仅接受 2450 MHz，RA/手机组给予 RA 加 915 MHz，RA/UHF 波组给予 RA 加 2450 MHz，RA 组给予 RA（20 mg/kg）。连续辐射 30 天后，测量其睾丸的生化和组织病理学参数。采用单因素方差分析（ANOVA），然后采用 Tukey's 检验进行统计学比较。
手机和 UHF 波辐射显著降低了抗氧化酶如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及谷胱甘肽含量（P<0.001）。相反，UHF 显著增加了氧化应激指标，包括丙二醛水平、一氧化氮水平和蛋白质羰基含量（P<0.001）。UHF 还显著减少了支持细胞、精原细胞、初级精母细胞、上皮高度以及生精小管和管腔直径（P<0.001）。RA 作为一种有效的抗氧化剂，通过显著降低氧化应激指标和抗氧化酶的升高，以及改善组织学参数，逆转了上述危害，并减轻了 UHF 对大鼠睾丸的不良影响（P<0.001）。
RA 可通过减少氧化应激来保护大鼠睾丸免受 UHF 诱导的毒性。RA 作为一种食品补充剂，可能有助于保护暴露于 UHF 环境污染的人类。
