Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Jagiellońska 4, 41-200 Sosnowiec, Poland.
Nutrients. 2019 Apr 9;11(4):803. doi: 10.3390/nu11040803.
Oxidative stress is believed to be associated with both postmenopausal disorders and cataract development. Previously, we have demonstrated that rosmarinic and sinapic acids, which are diet-derived antioxidative phenolic acids, counteracted some disorders induced by estrogen deficiency. Other studies have shown that some phenolic acids may reduce cataract development in various animal models. However, there is no data on the effect of phenolic acids on oxidative stress markers in the lenses of estrogen-deficient rats. The study aimed to investigate whether administration of rosmarinic acid and sinapic acid affects the antioxidative abilities and oxidative damage parameters in the lenses of estrogen-deficient rats. The study was conducted on three-month-old female Wistar rats. The ovariectomized rats were orally treated with rosmarinic acid at doses of 10 and 50 mg/kg or sinapic acid at doses of 5 and 25 mg/kg, for 4 weeks. The content of reduced glutathione (GSH), oxidized glutathione and amyloid β, as well as products of protein and lipid oxidation, were assessed. Moreover, the activities of superoxide dismutase, catalase, and some glutathione-related enzymes in the lenses were determined. Rosmarinic and sinapic acids in both doses resulted in an increase in the GSH content and glutathione reductase activity. They also improved parameters connected with protein oxidation. Since GSH plays an important role in maintaining the lens transparency, the increase in GSH content in lenses after the use of rosmarinic and sinapic acids seems to be beneficial. Therefore, both the investigated dietary compounds may be helpful in preventing cataract.
氧化应激被认为与绝经后疾病和白内障的发展有关。此前，我们已经证明，迷迭香酸和芥子酸这两种源自饮食的抗氧化酚酸可以对抗雌激素缺乏引起的一些疾病。其他研究表明，一些酚酸可能会减少各种动物模型中的白内障发展。然而，关于酚酸对雌激素缺乏大鼠晶状体中氧化应激标志物的影响，尚无数据。本研究旨在探讨迷迭香酸和芥子酸的给药是否会影响雌激素缺乏大鼠晶状体的抗氧化能力和氧化损伤参数。该研究在 3 月龄雌性 Wistar 大鼠上进行。去卵巢大鼠经口给予迷迭香酸 10 和 50mg/kg 或芥子酸 5 和 25mg/kg，连续 4 周。评估了还原型谷胱甘肽（GSH）、氧化型谷胱甘肽和淀粉样β的含量，以及蛋白质和脂质氧化产物的含量。此外，还测定了晶状体中超氧化物歧化酶、过氧化氢酶和一些谷胱甘肽相关酶的活性。两种剂量的迷迭香酸和芥子酸均导致 GSH 含量和谷胱甘肽还原酶活性增加。它们还改善了与蛋白质氧化相关的参数。由于 GSH 在维持晶状体透明性方面起着重要作用，因此使用迷迭香酸和芥子酸后晶状体中 GSH 含量的增加似乎是有益的。因此，这两种饮食化合物都可能有助于预防白内障。
