Seswandhana M R, Kurniawan I D, Anwar S L, Humani H M A, Gabriela G C, Dachlan I, Wirohadidjojo Y W, Aryandono I
Plastic, Reconstruction and Aesthetic Surgery Division, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Surgical Oncology Division, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia.
Ann Burns Fire Disasters. 2023 Sep 30;36(3):222-228. eCollection 2023 Sep.
Burn injuries are the fourth most common type of trauma worldwide, after traffic injuries, falls and interpersonal violence. Vascular endothelial growth factor (VEGF) is one of the most critical proangiogenic factors. Failure in angiogenesis is often associated with chronic, non-healing wounds. This study aimed to compare the effect of sterile gauze with normal saline (NaCl) 0.9%, intermittent negative pressure wound therapy (NPWT), continuous NPWT, and silver sulfadiazine dressing on increasing VEGF and angiogenesis in deep dermal burn injury. This experimental laboratory study involved six Yorkshire pigs. Twenty burns were made on each pig's flank and dorsum areas, which were divided into four treatment groups: sterile gauze with NaCl 0.9%, intermittent NPWT, continuous NPWT, and silver sulfadiazine dressing. Skin biopsies were done on days 1, 3, 7, 14 and 21 to evaluate VEGF histoscore and mean microvascular density (MVD). We used immunohistochemical staining of VEGF-165 as VEGF's protein marker and hematoxylin-eosin (HE) to count the MVD. There was a significant difference in mean VEGF histoscore on evaluation day 14, in which continuous NPWT had the highest score compared to sterile gauze with NaCl 0.9%, intermittent NPWT, and silver sulfadiazine. The elevated VEGF histoscore could significantly increase the MVD.
烧伤是全球第四大常见创伤类型，仅次于交通伤、跌倒和人际暴力。血管内皮生长因子（VEGF）是最关键的促血管生成因子之一。血管生成失败通常与慢性不愈合伤口有关。本研究旨在比较无菌纱布、0.9%生理盐水（NaCl）、间歇性负压伤口治疗（NPWT）、持续性NPWT和磺胺嘧啶银敷料对深度真皮烧伤中VEGF增加和血管生成的影响。这项实验性实验室研究涉及6只约克夏猪。在每只猪的侧腹和背部区域制造20处烧伤，将其分为四个治疗组：0.9% NaCl无菌纱布、间歇性NPWT、持续性NPWT和磺胺嘧啶银敷料。在第1、3、7、14和21天进行皮肤活检，以评估VEGF组织评分和平均微血管密度（MVD）。我们使用VEGF-165的免疫组织化学染色作为VEGF的蛋白质标记物，并使用苏木精-伊红（HE）染色来计数MVD。在评估第14天时，平均VEGF组织评分存在显著差异，其中持续性NPWT的评分高于0.9% NaCl无菌纱布、间歇性NPWT和磺胺嘧啶银。VEGF组织评分升高可显著增加MVD。
