Tang Xiaorong, Shi Jiahui, Lin Shumin, He Zhiyin, Cui Shuai, Di Wenhui, Chen Siyun, Wu Junshang, Yuan Si, Ye Qiuping, Yang Xiaoyun, Shang Ying, Zhang Zhaoxiang, Wang Lin, Lu Liming, Tang Chunzhi, Xu Nenggui, Yao Lulu
South China Research Center for Acupuncture and Moxibustion, Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Research Institute of Acupuncture and Meridian, Anhui University of Chinese Medicine, Hefei 230000, Anhui Province, China.
iScience. 2024 Apr 9;27(5):109695. doi: 10.1016/j.isci.2024.109695. eCollection 2024 May 17.
Electroacupuncture (EA) stimulation has been shown to be beneficial in stroke rehabilitation; however, little is known about the neurological mechanism by which this peripheral stimulation approach treats for stroke. This study showed that both pyramidal and parvalbumin (PV) neuronal activity increased in the contralesional primary motor cortex forelimb motor area (M1FL) after ischemic stroke induced by focal unilateral occlusion in the M1FL. EA stimulation reduced pyramidal neuronal activity and increased PV neuronal activity. These results were obtained by a combination of fiber photometry recordings, and electrophysiological recordings, and immunofluorescence. Moreover, EA was found to regulate the expression/function of N-methyl-D-aspartate receptors (NMDARs) altered by stroke pathology. In summary, our findings suggest that EA could restore disturbed neuronal activity through the regulation of the activity of pyramidal and PV neurons. Furthermore, NMDARs we shown to play an important role in EA-mediated improvements in sensorimotor ability during stroke rehabilitation.
电针刺激已被证明对中风康复有益；然而，对于这种外周刺激方法治疗中风的神经机制知之甚少。本研究表明，在局灶性单侧闭塞M1FL诱导的缺血性中风后，锥体神经元和小白蛋白（PV）神经元的活动在对侧初级运动皮层前肢运动区（M1FL）均增加。电针刺激降低了锥体神经元的活动，增加了PV神经元的活动。这些结果是通过光纤光度记录、电生理记录和免疫荧光相结合获得的。此外，发现电针可调节因中风病理改变的N-甲基-D-天冬氨酸受体（NMDARs）的表达/功能。总之，我们的研究结果表明，电针可通过调节锥体神经元和PV神经元的活动来恢复紊乱的神经元活动。此外，NMDARs在中风康复期间电针介导的感觉运动能力改善中发挥重要作用。
