Wang Jie, Zhang Shouke, Kong Junqia, Chang Jun
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
College of Landscape Architecture, Zhejiang A&F University, Hangzhou, China.
Front Microbiol. 2024 Apr 12;15:1379488. doi: 10.3389/fmicb.2024.1379488. eCollection 2024.
The plant secondary metabolites (PSMs), as important plant resistance indicators, are important targets for screening plant insect resistance breeding. In this study, we aimed to investigate whether the population of (ZC) is affected by different varieties of PSMs content. At the same time, the structure and function of the gut microbiome of ZC were also analyzed in relation to different pecan varieties.
We counted the populations of ZC larvae in four pecan varieties and determined the content of four types of PSMs. The structure and function of the larval gut microbiota were studied in connection to the number of larvae and the content of PSMs. The relationships were investigated between larval number, larval gut microbiota, and PSM content.
We found that the tannins, total phenolics, and total saponins of 4 various pecans PSMs stifled the development of the ZC larval population. The PSMs can significantly affect the diversity and abundance of the larval gut microbiota. Enrichment of ASV46 ( sp.), ASV994 ( sp.), ASV743 ( sp.), ASV709 ( sp.), ASV671 ( sp.), ASV599 ( sp.), ASV575 ( sp.), and ASV27 ( sp.) in the gut of larvae fed on high-resistance cultivars was positively associated with their tannin, total saponin, and total phenolic content. The results of the gut microbiome functional prediction for larvae fed highly resistant pecan varieties showed that the enriched pathways in the gut were related to the breakdown of hazardous chemicals.
Our findings provide further evidence that pecan PSMs influence the structure and function of the gut microbiota, which in turn affects the population stability of ZC. The study's findings can serve as a theoretical foundation for further work on selecting ZC-resistant cultivars and developing green management technology for ZC.
植物次生代谢产物（PSMs）作为重要的植物抗性指标，是筛选植物抗虫育种的重要目标。在本研究中，我们旨在探究不同品种PSMs含量是否会影响[某种昆虫名称（原文未完整给出）]（ZC）的种群数量。同时，还分析了与不同山核桃品种相关的ZC肠道微生物群的结构和功能。
我们统计了四个山核桃品种中ZC幼虫的数量，并测定了四种PSMs的含量。结合幼虫数量和PSMs含量研究了幼虫肠道微生物群的结构和功能。研究了幼虫数量、幼虫肠道微生物群和PSMs含量之间的关系。
我们发现4种不同山核桃PSMs中的单宁、总酚和总皂苷抑制了ZC幼虫种群的发育。PSMs能显著影响幼虫肠道微生物群的多样性和丰度。在高抗性品种上取食的幼虫肠道中，ASV46（[某种菌属名称（原文未完整给出）]）、ASV994（[某种菌属名称（原文未完整给出）]）、ASV743（[某种菌属名称（原文未完整给出）]）、ASV709（[某种菌属名称（原文未完整给出）]）、ASV671（[某种菌属名称（原文未完整给出）]）、ASV599（[某种菌属名称（原文未完整给出）]）、ASV575（[某种菌属名称（原文未完整给出）]）和ASV27（[某种菌属名称（原文未完整给出）]）的富集与它们的单宁、总皂苷和总酚含量呈正相关。对取食高抗性山核桃品种的幼虫肠道微生物群功能预测结果表明，肠道中富集的途径与有害化学物质的分解有关。
我们的研究结果进一步证明，山核桃PSMs会影响肠道微生物群的结构和功能，进而影响ZC的种群稳定性。该研究结果可为进一步开展ZC抗性品种筛选及ZC绿色防控技术研究提供理论依据。
