使用……可溶性表达重组凝血因子IX蛋白 。 （你提供的原文不完整，最后的using后面应该还有具体内容）
Soluble expression of recombinant coagulation factor IX protein using .
作者信息
Park Byoung-Hee, Yun Hanool, Jeong Hee-Jin
机构信息
Industry-Academia Cooperation Foundation, Hongik University, 2639 Sejong-ro, Sejong-si, 30016, South Korea.
Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Sejong-si, 30016, South Korea.
出版信息
Biochem Biophys Rep. 2024 Apr 18;38:101714. doi: 10.1016/j.bbrep.2024.101714. eCollection 2024 Jul.
Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using . We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity.
血友病B是一种由FIX缺乏引起的先天性出血性疾病。检测血友病B指标抗FIX抗体需要生成重组FIX（rFIX）。在本研究中，我们描述了一种使用[具体方式未给出]生产重组FIX（rFIX）的方法。我们构建了一个不含融合标签蛋白编码基因的FIX表达质粒，并在五天内以可溶形式生产出rFIX。使用抗FIX抗体通过ELISA获得了剂量依赖曲线，表明rFIX可用作抗原以高亲和力和灵敏度检测抗FIX抗体。
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