Jiao Shoude, Zhang Jing, Wang Jian, Ma Xueqing, Li Guoxiu, Li Jiaoyang, Cui Zhanding, Li Dong, Li Pinghua, Zeng Qiaoying, Liu Zaixin, Lu Zengjun, Sun Pu
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, China.
Front Vet Sci. 2024 Apr 12;11:1372032. doi: 10.3389/fvets.2024.1372032. eCollection 2024.
Porcine reproductive and respiratory syndrome virus (PRRSV) remains one of the major threats to swine industry, resulting in huge economic losses worldwide. Currently, PRRSV has diversified into multiple lineages with characteristics of extensive recombination in China. In this research, three virus strains were isolated and four virus whole genome sequences were generated and analyzed from clinical samples collected in Gansu province of China in 2023. The four virus strains were designated GSTS4-2023, GSLX2-2023, GSFEI2-2023 and GSBY4-2023. Phylogenetic analysis based on ORF5 sequences showed that GSTS4-2023, GSLX2-2023, GSFEI2-2023 and GSBY4-2023 shared 91.7, 91.2, 93.2 and 92.9% homology with NADC30 strain respectively, and belonged to lineage 1 of PRRSV-2. In addition, one amino acid deletion was observed at position 33 in ORF5 of GSTS4-2023, GSLX2-2023 and GSFEI2-2023. Moreover, amino acid alignment of the four strains showed a typical discontinuous 131-amino acid (aa) deletion in NSP2 for NADC30-like virus strains. Recombination analysis revealed that all four strains originated from NADC30 (lineage 1), with their minor parents coming from JXA1-like strains (lineage 8), VR-2332-like strains (lineage5) and QYYZ-like strains (lineage3). Finally, the three isolated virus strains, GSTS4-2023, GSLX2-2023 and GSFEI2-2023 showed relatively low levels of replication in cell culture. Our findings provide important implications for the field epidemiology of PRRSV.
猪繁殖与呼吸综合征病毒（PRRSV）仍然是养猪业的主要威胁之一，在全球范围内造成巨大经济损失。目前，PRRSV已在中国分化为多个谱系，具有广泛重组的特征。在本研究中，从2023年在中国甘肃省采集的临床样本中分离出三株病毒，并生成和分析了四个病毒全基因组序列。这四株病毒分别命名为GSTS4-2023、GSLX2-2023、GSFEI2-2023和GSBY4-2023。基于ORF5序列的系统发育分析表明，GSTS4-2023、GSLX2-2023、GSFEI2-2023和GSBY4-2023与NADC30毒株的同源性分别为91.7%、91.2%、93.2%和92.9%，属于PRRSV-2的1型谱系。此外，在GSTS4-2023、GSLX2-2023和GSFEI2-2023的ORF5第33位观察到一个氨基酸缺失。此外，这四株毒株的氨基酸比对显示，NSP2中存在典型的131个氨基酸（aa）的不连续缺失，这是NADC30样病毒毒株的特征。重组分析表明，所有四株毒株均起源于NADC30（1型谱系），其次要亲本分别来自JXA1样毒株（8型谱系）、VR-2332样毒株（5型谱系）和QYYZ样毒株（3型谱系）。最后，三株分离的病毒毒株GSTS4-2023、GSLX2-2023和GSFEI2-2023在细胞培养中的复制水平相对较低。我们的研究结果为PRRSV的现场流行病学提供了重要启示。
