Lozano Ana, Marrugo Victoria, Alvarado Juan Carlos, Hernandez Karen, Caballero Kevin Llinás, Acevedo Nathalie, Zakzuk Josefina, Caraballo Luis
Estudiante, Doctorado en Ciencias Biomédicas, Instituto de Investigaciones Inmunológicas, Universidad de Cartagena.
Asistente de investigación, Instituto de Investigaciones Inmunológicas, Universidad de Cartagena.
Rev Alerg Mex. 2024 Feb 1;71(1):69. doi: 10.29262/ram.v71i1.1372.
To compare the relative frequencies of immune cell populations in the peripheral blood according to infection status.
Peripheral blood samples were collected from participants infected (n = 35) and uninfected with (n=27) residing in different rural municipalities of Bolívar. Infection was diagnosed using two coprological examinations and the Kato-Katz technique. Immunophenotyping was performed using two panels of markers and staining in fresh blood. The flow cytometry reading was performed on a spectral cytometer (Northern Lights, Cytek, USA). The populations identified in the first panel (Figure 1) were T lymphocytes (CD45+ CD3+), CD4+ or CD8+, B lymphocytes (CD45+ SSClow CD3- CD19+), neutrophils (CD45+ SSChi CD3- CD16+), and eosinophils (CD45+ SSChi CD3- CD16low). Monocytes were identified in another panel (Figure 2): classical (CD14++ CD16 -), intermediate (CD14++ CD16+), and non-classical (CD14+ CD16++). Dendritic cells, including CD123 + + CD303 + (plasmacytoid), HLA-DR + + CD1c + (myeloid CD1c +), and CD14-CD141 + + (myeloid CD141 +), were also identified. The study received approval from the Ethics Committee of the University of Cartagena, and participants provided informed consent. Funding was provided by the Colombian Sistema General de Regalías under BPIN2020000100405 - BPIN2020000100364.
No significant differences were observed in age [mean cases: 35.69 (SD: 17.7) vs. controls: 37.04 (SD: 15.6) years] or sex (cases: 62.9% vs. controls: 74.1%) (Table 1). All infections were mild, with a median of 96 eggs (IQR, 48-216). A marginally significant difference was observed only in the percentage of neutrophils (45.37% in cases vs. 54.79% in controls, p=0.041) (Figure 3). Although the frequency of eosinophils was higher in the cases (8.1% vs. 6%), this difference was not significant (p=0.138) (Figure 3). No significant differences were observed in the populations of monocytes or dendritic cells between cases and controls (Figure 4).
Mild infection appears to affect the number of neutrophils in peripheral blood. The low infection intensity in the studied samples may explain the lack of a significant impact on other cellular populations.
根据感染状况比较外周血中免疫细胞群体的相对频率。
从居住在玻利瓦尔不同农村市镇的感染（n = 35）和未感染（n = 27）参与者中采集外周血样本。使用两种粪便检查和加藤-卡茨技术诊断感染。使用两组标志物对新鲜血液进行免疫表型分析和染色。流式细胞术读数在光谱细胞仪（美国Cytek公司的北极光）上进行。在第一组（图1）中鉴定出的群体为T淋巴细胞（CD45+ CD3+）、CD4+或CD8+、B淋巴细胞（CD45+ SSClow CD3- CD19+）、中性粒细胞（CD45+ SSChi CD3- CD16+）和嗜酸性粒细胞（CD45+ SSChi CD3- CD16low）。在另一组（图2）中鉴定出单核细胞：经典型（CD14++ CD16 -）、中间型（CD14++ CD16+）和非经典型（CD14+ CD16++）。还鉴定出树突状细胞，包括CD123 + + CD303 +（浆细胞样）、HLA-DR + + CD1c +（髓样CD1c +）和CD14-CD141 + +（髓样CD141 +）。该研究获得了卡塔赫纳大学伦理委员会的批准，参与者提供了知情同意书。资金由哥伦比亚皇家总系统根据BPIN2020000100405 - BPIN2020000100364提供。
在年龄[病例组均值：35.69（标准差：17.7）岁 vs. 对照组：37.04（标准差：15.6）岁]或性别（病例组：62.9% vs. 对照组：74.1%）方面未观察到显著差异（表1）。所有感染均为轻度，中位数为96个虫卵（四分位间距，48 - 216）。仅在中性粒细胞百分比方面观察到边缘显著差异（病例组为45.37%，对照组为54.79%，p = 0.041）（图3）。尽管病例组中嗜酸性粒细胞的频率较高（8.1% vs. 6%），但这种差异不显著（p = 0.138）（图3）。病例组和对照组之间在单核细胞或树突状细胞群体方面未观察到显著差异（图4）。
轻度感染似乎会影响外周血中中性粒细胞的数量。研究样本中感染强度较低可能解释了对其他细胞群体缺乏显著影响的原因。
