• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

核质转运速率受调节 GTP 可用性的细胞过程调控。

Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.

机构信息

Enabling Technologies Group, Sanford Research , Sioux Falls, SD, USA.

Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota , Vermillion, SD, USA.

出版信息

J Cell Biol. 2024 Jul 1;223(7). doi: 10.1083/jcb.202308152. Epub 2024 Apr 29.

DOI:10.1083/jcb.202308152
PMID:38683248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11059771/
Abstract

Nucleocytoplasmic transport (NCT), the facilitated diffusion of cargo molecules between the nucleus and cytoplasm through nuclear pore complexes (NPCs), enables numerous fundamental eukaryotic cellular processes. Ran GTPase uses cellular energy in the direct form of GTP to create a gradient across the nuclear envelope (NE) that drives the majority of NCT. We report here that changes in GTP availability resulting from altered cellular physiology modulate the rate of NCT, as monitored using synthetic and natural cargo, and the dynamics of Ran itself. Cell migration, cell spreading, and/or modulation of the cytoskeleton or its connection to the nucleus alter GTP availability and thus rates of NCT, regulating RNA export and protein synthesis. These findings support a model in which changes in cellular physiology that alter GTP availability can regulate the rate of NCT, impacting fundamental cellular processes that extensively utilize NCT.

摘要

核质转运（NCT），即通过核孔复合体（NPC）在细胞核和细胞质之间促进货物分子的扩散，使许多基本的真核细胞过程成为可能。Ran GTPase 利用细胞能量的直接形式 GTP 在核膜（NE）上产生梯度，从而驱动大多数 NCT。我们在这里报告说，改变细胞生理学导致的 GTP 可用性的变化会调节 NCT 的速率，这可以通过使用合成和天然货物以及 Ran 本身的动力学来监测。细胞迁移、细胞铺展以及/或细胞骨架或其与细胞核的连接的调节会改变 GTP 的可用性，从而改变 NCT 的速率，调节 RNA 输出和蛋白质合成。这些发现支持这样一种模型，即改变 GTP 可用性的细胞生理学变化可以调节 NCT 的速率，从而影响广泛利用 NCT 的基本细胞过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/edc0723ce961/JCB_202308152_Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/5a0e05723604/JCB_202308152_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/2b383a67f105/JCB_202308152_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/cf7b850b7229/JCB_202308152_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/38f9b5ed41f1/JCB_202308152_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/e52c3087b9f4/JCB_202308152_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/93af02ca2ba4/JCB_202308152_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/8675ab03433c/JCB_202308152_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/d5ad159b7fa2/JCB_202308152_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/f03c4354bc6a/JCB_202308152_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/600caa4d1b4d/JCB_202308152_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/014b952a3974/JCB_202308152_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/5563e0c61c14/JCB_202308152_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/dd5c9b700e74/JCB_202308152_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/8ea8b45f357b/JCB_202308152_Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/edc0723ce961/JCB_202308152_Fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/5a0e05723604/JCB_202308152_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/2b383a67f105/JCB_202308152_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/cf7b850b7229/JCB_202308152_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/38f9b5ed41f1/JCB_202308152_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/e52c3087b9f4/JCB_202308152_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/93af02ca2ba4/JCB_202308152_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/8675ab03433c/JCB_202308152_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/d5ad159b7fa2/JCB_202308152_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/f03c4354bc6a/JCB_202308152_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/600caa4d1b4d/JCB_202308152_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/014b952a3974/JCB_202308152_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/5563e0c61c14/JCB_202308152_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/dd5c9b700e74/JCB_202308152_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/8ea8b45f357b/JCB_202308152_Fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c55/11059771/edc0723ce961/JCB_202308152_Fig10.jpg

相似文献

1
Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.核质转运速率受调节 GTP 可用性的细胞过程调控。
J Cell Biol. 2024 Jul 1;223(7). doi: 10.1083/jcb.202308152. Epub 2024 Apr 29.
2
Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.核质运输速率受调节GTP可用性的细胞过程调控。
bioRxiv. 2023 Dec 30:2023.12.29.573651. doi: 10.1101/2023.12.29.573651.
3
On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions.核质转运的不对称分配——最新见解与待解问题。
J Cell Sci. 2021 Apr 1;134(7). doi: 10.1242/jcs.240382. Epub 2021 Apr 13.
4
Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability.核孔复合体处的核输入蛋白富集减弱了 Ran 的通透性。
J Cell Sci. 2020 Feb 12;133(3):jcs238121. doi: 10.1242/jcs.238121.
5
The bioenergetics of nucleocytoplasmic transport.核质转运的生物能量学。
J Cell Biol. 2024 Jul 1;223(7). doi: 10.1083/jcb.202405121. Epub 2024 Jun 7.
6
Systems analysis of Ran transport.Ran转运的系统分析
Science. 2002 Jan 18;295(5554):488-91. doi: 10.1126/science.1064732.
7
Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export.Ran结合蛋白3是Crm1介导的核蛋白输出的辅助因子。
J Cell Biol. 2001 Jun 25;153(7):1391-402. doi: 10.1083/jcb.153.7.1391.
8
Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1.Ran结合蛋白RanBP1的核质穿梭促进作用。
Mol Cell Biol. 2000 May;20(10):3510-21. doi: 10.1128/MCB.20.10.3510-3521.2000.
9
Influence of cargo size on Ran and energy requirements for nuclear protein import.货物大小对Ran及核蛋白输入能量需求的影响。
J Cell Biol. 2002 Oct 14;159(1):55-67. doi: 10.1083/jcb.200204163. Epub 2002 Oct 7.
10
Cex1p facilitates Rna1p-mediated dissociation of the Los1p-tRNA-Gsp1p-GTP export complex.Cex1p 促进了 Los1p-tRNA-Gsp1p-GTP 外排复合物由 RNA1p 介导的解离。
Traffic. 2012 Feb;13(2):234-56. doi: 10.1111/j.1600-0854.2011.01304.x. Epub 2011 Nov 15.

引用本文的文献

1
A tubulin-binding protein that preferentially binds to GDP-tubulin and promotes GTP exchange.一种微管蛋白结合蛋白，优先结合GDP-微管蛋白并促进GTP交换。
J Biol Chem. 2025 Jun 19;301(8):110401. doi: 10.1016/j.jbc.2025.110401.
2
The Complexities of Interspecies Somatic Cell Nuclear Transfer: From Biological and Molecular Insights to Future Perspectives.种间体细胞核移植的复杂性：从生物学和分子学见解到未来展望
Int J Mol Sci. 2025 Apr 2;26(7):3310. doi: 10.3390/ijms26073310.
3
A tubulin-binding protein that preferentially binds to GDP-tubulin and promotes GTP exchange.

本文引用的文献

1
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.SUN2 中的二硫键调节核膜上 LINC 复合物的动态重塑。
Life Sci Alliance. 2023 May 15;6(8). doi: 10.26508/lsa.202302031. Print 2023 Aug.
2
The Role of Bioenergetics in Neurodegeneration.生物能量学在神经退行性变中的作用。
Int J Mol Sci. 2022 Aug 16;23(16):9212. doi: 10.3390/ijms23169212.
3
Mechanical force application to the nucleus regulates nucleocytoplasmic transport.机械力施加于核上调节核质转运。
一种微管蛋白结合蛋白，它优先结合GDP-微管蛋白并促进GTP交换。
bioRxiv. 2024 Jul 15:2023.05.09.539990. doi: 10.1101/2023.05.09.539990.
Nat Cell Biol. 2022 Jun;24(6):896-905. doi: 10.1038/s41556-022-00927-7. Epub 2022 Jun 9.
4
Nuclear pores dilate and constrict in cellulo.核孔在细胞内扩张和收缩。
Science. 2021 Dec 10;374(6573):eabd9776. doi: 10.1126/science.abd9776.
5
Regulation of local GTP availability controls RAC1 activity and cell invasion.局部 GTP 可用性的调节控制 RAC1 活性和细胞侵袭。
Nat Commun. 2021 Oct 19;12(1):6091. doi: 10.1038/s41467-021-26324-6.
6
Nucleocytoplasmic Transport: Regulatory Mechanisms and the Implications in Neurodegeneration.核质转运：调控机制及其在神经退行性疾病中的意义。
Int J Mol Sci. 2021 Apr 17;22(8):4165. doi: 10.3390/ijms22084165.
7
On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions.核质转运的不对称分配——最新见解与待解问题。
J Cell Sci. 2021 Apr 1;134(7). doi: 10.1242/jcs.240382. Epub 2021 Apr 13.
8
Fueling the cytoskeleton - links between cell metabolism and actin remodeling.为细胞骨架供能——细胞代谢与肌动蛋白重构之间的联系。
J Cell Sci. 2021 Feb 8;134(3):jcs248385. doi: 10.1242/jcs.248385.
9
Structure, Folding and Stability of Nucleoside Diphosphate Kinases.核苷二磷酸激酶的结构、折叠和稳定性。
Int J Mol Sci. 2020 Sep 16;21(18):6779. doi: 10.3390/ijms21186779.
10
Mechanical stress triggers nuclear remodeling and the formation of transmembrane actin nuclear lines with associated nuclear pore complexes.机械应力引发核重塑，并形成带有相关核孔复合体的跨膜肌动蛋白核线。
Mol Biol Cell. 2020 Jul 21;31(16):1774-1787. doi: 10.1091/mbc.E19-01-0027. Epub 2020 Jan 22.