Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
National Botanic Garden of Wales, Llanarthne, UK.
Methods Mol Biol. 2024;2744:445-473. doi: 10.1007/978-1-0716-3581-0_27.
Plant DNA barcoding has a multitude of applications ranging from species detection and biomonitoring to investigating ecological networks and checking food quality. The ability to accurately identify species, using DNA barcoding, depends on the quality and comprehensiveness of the reference library that is used. This chapter describes how to create plant reference libraries using the rbcL, matK, and ITS2 DNA barcode regions. It covers the creation of species lists, the collection of specimens from the field and herbarium, DNA extraction, PCR amplification, and DNA sequencing. This methodology gives special attention to using samples from herbaria, as they represent important collections of easily accessible, taxonomically verified plant material.
植物 DNA 条形码技术在物种检测、生物监测、生态网络研究以及食品质量检查等方面具有广泛的应用。使用 DNA 条形码准确识别物种的能力取决于所使用的参考文库的质量和全面性。本章介绍了如何使用 rbcL、matK 和 ITS2 DNA 条形码区域创建植物参考文库。它涵盖了创建物种清单、从野外和标本馆采集标本、DNA 提取、PCR 扩增和 DNA 测序等步骤。该方法特别关注使用标本馆的样本，因为它们是易于获取且经分类学验证的植物材料的重要收藏。
