G.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Medical Center-Albert-Ludwigs-University of Freiburg, Albert-Ludwigs- University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany.
Robert Mathys Foundation RMS, Bischmattstr. 12, Bettlach, 2544, Switzerland.
BMC Res Notes. 2024 Apr 29;17(1):122. doi: 10.1186/s13104-024-06781-0.
Fluorescence analysis of β-TCP ceramics is often used to describe cells found on said ceramics. However, we found, to our knowledge, so far undescribed artifacts which might sometimes be hard to differentiate from cells due to shape and fluorescence behavior. We tried prolonged ultrasound washing as well as Technovit 9100 fixation to reduce these artifacts. While untreated dowels showed no reduction in artifacts no matter the further treatment, Technovit fixation reduced the artifacts with even further reduction achieved by mechanical cleaning. As a consequence, scientists working with these dowels and likely even other types should try to avoid creating false positive results by considering the existence of these artifacts, checking additional filters for unusual fluorescence and by reducing them by using Technovit fixation when possible.
β-TCP 陶瓷的荧光分析常用于描述在陶瓷上发现的细胞。然而，据我们所知，到目前为止，我们发现了一些尚未描述的伪影，由于形状和荧光行为，这些伪影有时可能很难与细胞区分开来。我们尝试了长时间的超声清洗和 Technovit 9100 固定，以减少这些伪影。虽然未经处理的插杆无论进一步如何处理都没有减少伪影，但 Technovit 固定减少了伪影，通过机械清洁甚至进一步减少了伪影。因此，使用这些插杆的科学家，甚至可能是其他类型的科学家，应该通过考虑这些伪影的存在、检查异常荧光的其他滤波器以及在可能时使用 Technovit 固定来减少它们，以避免产生假阳性结果。
