Laboratory of Synthetic Biology, Department of Bioengineering, School of Life Science and Technology, Xinjiang University, Urumqi, 830017, People's Republic of China; School of Future Technology, Xinjiang University, Urumqi, 830017, People's Republic of China.
Laboratory of Synthetic Biology, Department of Bioengineering, School of Life Science and Technology, Xinjiang University, Urumqi, 830017, People's Republic of China.
Environ Res. 2024 Jul 1;252(Pt 3):119010. doi: 10.1016/j.envres.2024.119010. Epub 2024 Apr 27.
The disposal of animal remains resulting from breeding is a significant challenge that impacts the industry's growth. To address the issues with current treatment methods, such as the large space required for corpse storage, and the high energy consumption of pyrolysis. Three strains with high protease and lipase production and one strain with high keratinase production were screened. The virulence genes were evaluated, the synthesis gene clusters of degrading enzymes were mined, secondary metabolites of each strain were analyzed, and the bacterial community with both growth rate and enzyme production ability was developed. Therefore, a microbial degradation method with mild reaction conditions and rapid liquefaction of animal residues was developed. The liquid degradation of four common farm-raised animal residues (sheep, cattle, chickens, and pigs) was tested under laboratory conditions. The results showed that the liquid degradation of animal residues was achieved within 144 h, transforming the months-long anaerobic process of traditional compost fermentation process into a mere 6 days' anaerobic process. N, P, K plant nutrients accounted for 15% of the total matrix, pH value was 5.5-6.7, heavy metal content was less than 0.2 mg L. Designed and improved fermentation equipment, produced a 3 m³ fermentation equipment, used in chicken, pig two types of animal residues pilot test. The emissions of greenhouse gases such as CO in the entire degradation process were 1.6 × 10 ppm, which was 481 times less than that of composting by 7.7 × 10. This study provides a solution for the treatment of dead livestock and poultry, which has promotional and practical value.
养殖动物遗体的处理是一个重大挑战,影响着该行业的发展。为了解决当前处理方法存在的问题,如尸体储存需要大量空间和热解需要高能耗等问题,筛选出三株产蛋白酶和脂肪酶能力较高的菌株和一株产角蛋白酶能力较高的菌株。评估了其毒力基因,挖掘了降解酶的合成基因簇,分析了各菌株的次生代谢物,并开发出具有生长速度和产酶能力的细菌群落。因此,开发了一种具有温和反应条件和快速液化动物残余物的微生物降解方法。在实验室条件下,测试了四种常见养殖动物(绵羊、牛、鸡和猪)残余物的液体降解情况。结果表明,动物残余物的液体降解在 144 小时内完成,将传统堆肥发酵过程中数月的厌氧过程缩短至仅 6 天的厌氧过程。N、P、K 植物养分占总基质的 15%,pH 值为 5.5-6.7,重金属含量小于 0.2 mg L。设计并改进了发酵设备,生产了 3 立方米发酵设备,用于鸡、猪两种动物残余物的中试。在整个降解过程中,温室气体如 CO 的排放量为 1.6×10 ppm,比堆肥的 7.7×10 少 481 倍。本研究为处理病死畜禽提供了一种解决方案,具有推广和实用价值。