Laboratory of Applied Biotechnology, Clinical Hospital of the Medical School, São Paulo State University (UNESP), Botucatu, Brazil.
Laboratory of Pharmaceutical Processes/LAPROFAR, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
J Microencapsul. 2024 Jun;41(4):284-295. doi: 10.1080/02652048.2024.2348463. Epub 2024 Apr 30.
This work aimed to investigate the effectiveness of and essential oils (EOs) encapsulated in nanostructured lipid carriers (NLCs) as SARS-CoV-2 inhibitors through virucidal activity assessment. We developed anionic and cationic NLCs loaded with the EOs and assessed their physicochemical properties and SARS-CoV-2 virucidal activity, focusing on the effects of EO type and the NLCs composition. The NLCs exhibited particle sizes of 141.30 to 160.53 nm for anionic and 109.30 to 138.60 nm for cationic types, with PDIs between 0.16 and 0.25. High zeta potentials (>29.0 in modulus) indicated stable formulations. The NLCs effectively encapsulated the EOs, achieving encapsulation efficiencies between 84.6 to 100% w/w of marker compound. The EOs-loaded NLCs reduced the SARS-CoV-2 virion count, exceeding 2 logs over the control. NLCs loaded with and EOs represent an innovative strategy for combating SARS-CoV-2.
本研究旨在通过评估病毒杀灭活性，探究负载于纳米结构脂质载体（NLC）中的 和 精油作为 SARS-CoV-2 抑制剂的效果。我们制备了负载有精油的阴离子型和阳离子型 NLC，并评估了它们的理化性质和 SARS-CoV-2 病毒杀灭活性，重点关注了精油类型和 NLC 组成的影响。NLC 的粒径为阴离子型 141.30 至 160.53nm，阳离子型 109.30 至 138.60nm，PDI 在 0.16 至 0.25 之间。高 Zeta 电位（模数>29.0）表明了制剂的稳定性。NLC 能够有效地包封精油，实现了标记化合物 84.6%至 100%w/w 的包封效率。负载精油的 NLC 降低了 SARS-CoV-2 病毒粒子的数量，与对照组相比，超过了 2 个对数级。负载 和 精油的 NLC 为抗击 SARS-CoV-2 提供了一种创新策略。
