Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, School of Material Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Augustana Faculty, University of Alberta, Camrose T4V 2R3, Canada.
ACS Appl Mater Interfaces. 2024 May 15;16(19):24771-24780. doi: 10.1021/acsami.4c02788. Epub 2024 Apr 30.
Photosynthetic inorganic biohybrid systems (PBSs) combining an inorganic photosensitizer with intact living cells provide an innovative view for solar hydrogen production. However, typical whole-cell biohybrid systems often suffer from sluggish electron transfer kinetics during transmembrane diffusion, which severely limits the efficiency of solar hydrogen production. Here, a unique biohybrid system with a quantum yield of 8.42% was constructed by feeding bismuth-doped carbon dots (Bi@CD) to (). In this biohybrid system, Bi@CD can enter the cells and transfer the electrons upon light irradiation, greatly reducing the energy loss and shortening the distance of electron transfer. More importantly, the photocatalytic hydrogen production of the -Bi@CDs biohybrid system reached up to 0.95 mmol within 3 h under light irradiation (420-780 nm, 2000 W m), which is 1.36 and 2.38 times higher than that in the -CDs biohybrid system and the system, respectively. In addition, the mechanism of enhanced hydrogen production was further explored. It was found that the accelerated decomposition of glucose, the accelerated production of pyruvate, the inhibition of lactic acid, and the increase of formic acid were the reasons for the increase of hydrogen production. This work provides a novel strategy for improving the hydrogen production in photosynthetic inorganic biohybrid systems.
光合无机生物杂化系统（PBS）将无机光敏剂与完整的活细胞结合，为太阳能制氢提供了新的视角。然而，典型的全细胞生物杂化系统在跨膜扩散过程中往往存在电子转移动力学缓慢的问题，这严重限制了太阳能制氢的效率。在这里，通过向 () 中添加掺铋碳点 (Bi@CD) 构建了一种独特的量子产率为 8.42%的生物杂化系统。在该生物杂化系统中，Bi@CD 可以在光照下进入细胞并转移电子，从而大大减少能量损失并缩短电子转移距离。更重要的是，在光照下（420-780nm，2000W m），-Bi@CDs 生物杂化系统的光催化制氢在 3 小时内达到了 0.95mmol，分别是 -CDs 生物杂化系统和 系统的 1.36 倍和 2.38 倍。此外，进一步探讨了增强制氢的机制。研究发现，葡萄糖的加速分解、丙酮酸的加速产生、乳酸的抑制和甲酸的增加是产氢增加的原因。这项工作为提高光合无机生物杂化系统的制氢效率提供了一种新策略。
