Zhang Haiguang, Wang Yuping, Hu Qingxi, Liu Qiong
Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai, China.
Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai, China.
3D Print Addit Manuf. 2024 Apr 1;11(2):e607-e618. doi: 10.1089/3dp.2022.0111. Epub 2024 Apr 16.
Large bone defects caused by congenital deformities and acquired accidents are increasing day by day. A large number of patients mainly rely on artificial bone for repair. However, artificial bone cannot fully imitate the structure and composition of human bone, resulting in a large gap with autologous bone function. Therefore, this article proposes a continuous preparation method for inorganic/organic biphasic composite gradient biomimetic bulk bone scaffolds. First, a controllable gradient hybrid forming platform for inorganic/organic dual-phase biomaterials was constructed, and the feeding control strategy was studied to achieve precise control of the feeding of sodium alginate/gelatin composite organic materials and hydroxyapatite inorganic materials. The speed is, respectively, sent from the corresponding feeding nozzle to the mixing chamber to realize the uniform mixing of the biphasic material and the extrusion of the composite material, and the inorganic/organic biphasic composite gradient biomimetic bone scaffold with gradual structure and composition is prepared. Second, to prove the superiority of the preparation method, the physicochemical and biological properties of the prepared scaffolds were evaluated. The test results showed that the morphological characteristics of the biphasic composite gradient bone scaffold showed good microscopic porosity and the structure and composition showed gradients. The mechanical properties are close to that of human bone tissue and cell experiments show that the scaffold has good biocompatibility and bioactivity. In conclusion, this article provides a new type of bone scaffold preparation technology and equipment for the field of tissue engineering, which has research value and application prospects.
由先天性畸形和后天事故导致的大骨缺损日益增多。大量患者主要依靠人工骨进行修复。然而，人工骨无法完全模仿人体骨骼的结构和组成，导致其与自体骨功能存在较大差距。因此，本文提出一种无机/有机双相复合梯度仿生块状骨支架的连续制备方法。首先，构建了无机/有机双相生物材料可控梯度混合成型平台，并研究了进料控制策略，以实现对海藻酸钠/明胶复合有机材料和羟基磷灰石无机材料进料的精确控制。将其分别从相应的进料喷嘴输送至混合腔，实现双相材料的均匀混合以及复合材料的挤出，制备出结构和组成呈梯度变化的无机/有机双相复合梯度仿生骨支架。其次，为证明该制备方法的优越性，对所制备支架的物理化学和生物学性能进行了评估。测试结果表明，双相复合梯度骨支架的形态特征显示出良好的微观孔隙率，其结构和组成呈现梯度变化。力学性能接近人体骨组织，细胞实验表明该支架具有良好的生物相容性和生物活性。总之，本文为组织工程领域提供了一种新型的骨支架制备技术及设备，具有研究价值和应用前景。
