唾液蛋白酶 3 作为儿童龋齿严重程度的生物标志物：一项横断面研究。
Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study.
机构信息
Department of Pediatric and Preventive Dentistry, PMS College of Dental Science & Research, Vattappara, Thiruvananthapuram, Kerala, India.
Department of Oral Medicine and Radiology, PMS College of Dental Science & Research, Vattappara, Thiruvananthapuram, Kerala, India.
出版信息
J Contemp Dent Pract. 2024 Mar 19;25(3):236-240. doi: 10.5005/jp-journals-10024-3648.
AIM
This study aims to evaluate the relation between salivary proteinase 3 (PR3) concentration and caries severity in children.
MATERIALS AND METHODS
Six-to-eight-year age group children, from the Outpatient Department of Pediatric and Preventive dentistry at PMS Dental College were selected for the study. From these children, three groups each consisting of 28 children were selected according to the dental caries severity. Three groups were: (1) No Dental Caries group, (2) Low Dental Caries group with DMFT/DEFT score of 1-4, and (3) High Dental Caries group with DMFT/DEFT score of 5-15. Thus, a total of 84 children who satisfied the inclusion criteria were selected. The concentration of PR3 in saliva of the donors were analyzed using an ELISA kit. One way ANOVA was used for finding the relation of salivary PR3 concentration with caries severity. Pairwise comparison of PR3 concentration and caries severity were analyzed using Tukey test.
RESULTS
Severity of caries and concentration of salivary PR3 showed an inverse relation. As the caries severity increases there was a decrease in PR3 concentration and vice versa.
CONCLUSION
The children with high caries severity showed lower concentration of PR3 in their saliva compared with those with lower caries severity which indicates that PR3 can be used as a biomarker for assessing caries severity and also paves way to use PR3 as a caries vaccine in future. Nowadays, interest toward noninvasive and personalized dentistry has been increased. Molecular assays using salivary biomarkers can be an effective tool in detecting the caries in earlier stages and assessing a patient's caries risk.
CLINICAL SIGNIFICANCE
Salivary PR3 can be used as prognostic biomarker for assessing caries severity and after treatment the value of PR3 can be used as a assessment tool to confirm its relation with caries. How to cite this article: Karthika S, George S, Soman A, . Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study. J Contemp Dent Pract 2024;25(3):236-240.
目的
本研究旨在评估唾液蛋白酶 3（PR3）浓度与儿童龋齿严重程度的关系。
材料和方法
选择 PMS 牙科医学院儿牙科和预防牙科门诊的 6-8 岁年龄组儿童进行研究。从这些儿童中，根据龋齿严重程度，选择每组各 28 名儿童的三个组。三组分别为：（1）无龋齿组，（2）DMFT/DEFT 评分 1-4 的低龋齿组，和（3）DMFT/DEFT 评分 5-15 的高龋齿组。因此，共选择了 84 名符合纳入标准的儿童。使用 ELISA 试剂盒分析供体唾液中 PR3 的浓度。使用单因素方差分析（ANOVA）来发现唾液 PR3 浓度与龋齿严重程度的关系。使用 Tukey 检验分析 PR3 浓度与龋齿严重程度的两两比较。
结果
龋齿严重程度和唾液 PR3 浓度呈负相关。随着龋齿严重程度的增加，PR3 浓度降低，反之亦然。
结论
与龋齿严重程度较低的儿童相比，高龋齿严重程度的儿童唾液中 PR3 的浓度较低，这表明 PR3 可用作评估龋齿严重程度的生物标志物，也为将来将 PR3 用作龋齿疫苗铺平了道路。如今，人们对非侵入性和个性化牙科的兴趣有所增加。使用唾液生物标志物的分子检测可以成为检测早期龋齿和评估患者龋齿风险的有效工具。
临床意义
唾液 PR3 可作为评估龋齿严重程度的预后生物标志物，治疗后，PR3 的值可用作评估工具，以确认其与龋齿的关系。
如何引用本文
Karthika S, George S, Soman A,. Salivary Proteinase 3 as a Biomarker for Caries Severity in Children: A Cross-sectional Study. J Contemp Dent Pract 2024;25(3):236-240.
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