Department of Dentistry for Children, Pediatric Dentistry Area, Piracicaba Dental School, University of Campinas Piracicaba, Brazil.
Caries Res. 2017;51(4):394-401. doi: 10.1159/000470849. Epub 2017 Jun 21.
This study aimed to analyze the concentration and activity of carbonic anhydrase (CA) VI in the saliva of school children. We investigated the relationship among caries, CA VI concentration/activity, flow rate, pH, and buffering capacity.
Seventy-four school children were divided into a caries-free group and a caries group. Clinical examinations were conducted by one examiner according to World Health Organization criteria + early caries lesions. Salivary flow rate, pH, and buffering capacity were analyzed. Salivary CA VI concentration and activity were evaluated by ELISA and zymography, respectively. The data were analyzed using Student's t test and the Mann-Whitney test, and Pearson and Spearman correlation analyses were also done. In multivariate modeling, associations between variables were expressed as odds ratios.
The results showed that salivary flow rate, salivary pH, and BC were significantly higher in the saliva of caries-free children. Also, the salivary CA VI concentration was significantly higher in the saliva of caries-free children. The salivary CA VI activity was higher in children with caries. We found a negative correlation between BC and dental caries. Also, in the caries group we found a positive correlation between the concentration and the activity of CA VI and a negative correlation between BC and CA VI activity. A negative correlation between salivary pH and CA VI concentration was observed in the caries-free group. A high activity of CA and a low salivary flow rate were associated with dental caries.
These results support the conclusion that dental caries is highly affected by the activity of CA VI in saliva as well as by the salivary flow rate.
本研究旨在分析儿童唾液中碳酸酐酶（CA）VI 的浓度和活性。我们研究了龋齿、CA VI 浓度/活性、流速、pH 值和缓冲能力之间的关系。
74 名学龄儿童分为无龋组和龋病组。临床检查由一名检查者根据世界卫生组织标准+早期龋损进行。分析唾液流速、pH 值和缓冲能力。通过 ELISA 和同工酶分析评估唾液 CA VI 浓度和活性。使用 Student's t 检验和 Mann-Whitney 检验分析数据，并进行 Pearson 和 Spearman 相关性分析。在多变量模型中，变量之间的关联表示为比值比。
结果表明，无龋儿童的唾液流速、唾液 pH 值和 BC 显著较高。此外，无龋儿童的唾液 CA VI 浓度也显著较高。龋病儿童的唾液 CA VI 活性较高。我们发现 BC 与龋齿之间存在负相关。此外，在龋病组中，我们发现 CA VI 浓度和活性之间存在正相关，BC 与 CA VI 活性之间存在负相关。在无龋组中观察到唾液 pH 值与 CA VI 浓度之间存在负相关。高 CA 活性和低唾液流速与龋齿有关。
这些结果支持结论，即龋齿受唾液中 CA VI 的活性以及唾液流速的强烈影响。
