College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Bioresour Technol. 2024 Jun;402:130767. doi: 10.1016/j.biortech.2024.130767. Epub 2024 Apr 30.
The study assessed the effect of salinity and lead (Pb(II)) on the anammox sludge for nitrogen removal from saline wastewater. Results showed decreased nitrogen removal and specific anammox activity (SAA) with elevated salinity and Pb(II). SAA reduced from 541.3 ± 4.3 mg N g VSS d at 0.5 mg/L Pb(II) to 436.0 ± 0.2 mg N g VSS d at 30 g/L NaCl, further to 303.6 ± 7.1 mg N g VSS d under 30 g/L NaCl + 0.5 mg/L Pb(II). Notably, the combined inhibition at salinity (15-20 g/L NaCl) and Pb(II) (0.3-0.4 mg/L) exhibited synergistic effect, while higher salinity and Pb(II) aligned with independent inhibition models. Combined inhibition decreased protein/polysaccharides ratio, indicating more severe negative effect on anammox aggregation capacity. Metagenomics confirmed decreased Candidatus Kuenenia, and enhanced denitrification under elevated salinity and Pb(II) conditions. This study offers insights into anammox operation for treating saline wastewater with heavy metals.
该研究评估了盐度和铅（Pb（II））对用于从含盐废水中去除氮的厌氧氨氧化污泥的影响。结果表明，随着盐度和 Pb（II）的升高，氮去除率和特定厌氧氨氧化活性（SAA）降低。SAA 从 0.5mg/L Pb（II）下的 541.3±4.3mgN/g VSS d 降低到 30g/L NaCl 下的 436.0±0.2mgN/g VSS d，在 30g/L NaCl+0.5mg/L Pb（II）下进一步降低至 303.6±7.1mgN/g VSS d。值得注意的是，盐度（15-20g/L NaCl）和 Pb（II）（0.3-0.4mg/L）的联合抑制表现出协同效应，而较高的盐度和 Pb（II）则符合独立抑制模型。联合抑制降低了蛋白质/多糖比，表明对厌氧氨氧化聚集能力的负面影响更严重。宏基因组学证实，在升高的盐度和 Pb（II）条件下，Candidatus Kuenenia 的丰度降低，反硝化作用增强。本研究为处理含重金属的含盐废水的厌氧氨氧化运行提供了见解。
