Lin Hazel, Del Rio Castillo Antonio Esau, González Viviana Jehová, Bonaccorso Francesco, Vázquez Ester, Fadeel Bengt, Bianco Alberto
CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR 3572, University of Strasbourg, ISIS 67000 Strasbourg France
BeDimensional Lungo Torrente Secca 30r Genoa Italy.
Nanoscale Adv. 2024 Mar 29;6(9):2419-2430. doi: 10.1039/d3na00863k. eCollection 2024 Apr 30.
Molybdenum disulfide is an emerging 2D material with several potential applications in medicine. Therefore, it is crucial to ascertain its biocompatibility. Mast cells are immune cells that are found in many organs and tissues in contact with the extracellular environment, and can be cultured from progenitor cells present in the bone marrow. Given the long period required for differentiation and proliferation of primary mast cells, human mast cell lines have emerged as a tractable model for biological and toxicological studies. Here, we compare two types of industrial MoS using CD34-derived primary human mast cells and the LAD2 cell line. Minimal effects were observed on early-stage activation endpoints such as β-hexosaminidase release and expression of surface markers of mast cell activation. Transmission electron microscopy revealed limited uptake of the tested materials. Overall, MoS was found to be biocompatible, and the LAD2 cell line was validated as a useful model of mast cells.
二硫化钼是一种新兴的二维材料，在医学领域有多种潜在应用。因此，确定其生物相容性至关重要。肥大细胞是一种免疫细胞，存在于许多与细胞外环境接触的器官和组织中，并且可以从骨髓中的祖细胞培养而来。鉴于原代肥大细胞分化和增殖所需的时间较长，人类肥大细胞系已成为生物学和毒理学研究的一种易于处理的模型。在这里，我们使用源自CD34的原代人类肥大细胞和LAD2细胞系比较了两种工业用二硫化钼。在早期激活终点，如β-己糖胺酶释放和肥大细胞激活表面标志物的表达方面，观察到的影响极小。透射电子显微镜显示受试材料的摄取有限。总体而言，发现二硫化钼具有生物相容性，并且LAD2细胞系被验证为肥大细胞的有用模型。
