Peerapen Paleerath, Putpeerawit Pattaranit, Boonmark Wanida, Thongboonkerd Visith
Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Curr Res Food Sci. 2024 Apr 18;8:100740. doi: 10.1016/j.crfs.2024.100740. eCollection 2024.
Resveratrol is a natural phenolic compound that belongs to stilbenoid group found in diverse plants. Health benefits and therapeutic potentials of resveratrol have been widely recognized in various diseases. In kidney stone disease, it can alleviate oxalate-induced hyperproduction of free radicals in renal epithelial cells. Nevertheless, its direct effects on calcium oxalate (CaOx) crystal, which is the major stone component, remained unclear. This study therefore addressed the direct effects of resveratrol (at 1, 10 or 100 μM) on each step of CaOx kidney stone formation. The results revealed that resveratrol had no significant effects on CaOx crystallization. However, resveratrol significantly decreased CaOx crystal growth and adhesion to renal epithelial cells at all concentrations, and induced crystal internalization into the cells (a process related to crystal degradation by endolysosomes) in a concentration-dependent manner. On the other hand, resveratrol promoted crystal aggregation. These data indicate that resveratrol serves as a dual modulator on CaOx stone formation. While it inhibits CaOx stone development by reducing crystal growth and adhesion to renal cells and by inducing crystal internalization into the cells, resveratrol promotes crystal aggregation, which is one of the mechanisms leading to kidney stone formation.
白藜芦醇是一种天然酚类化合物，属于在多种植物中发现的芪类化合物。白藜芦醇的健康益处和治疗潜力在各种疾病中已得到广泛认可。在肾结石疾病中，它可以减轻草酸盐诱导的肾上皮细胞中自由基的过度产生。然而，其对草酸钙（CaOx）晶体（肾结石的主要成分）的直接作用仍不清楚。因此，本研究探讨了白藜芦醇（1、10或100μM）对CaOx肾结石形成各步骤的直接作用。结果显示，白藜芦醇对CaOx结晶没有显著影响。然而，白藜芦醇在所有浓度下均显著降低CaOx晶体生长以及晶体与肾上皮细胞的粘附，并以浓度依赖的方式诱导晶体内化进入细胞（这一过程与溶酶体对晶体的降解有关）。另一方面，白藜芦醇促进晶体聚集。这些数据表明，白藜芦醇是CaOx结石形成的双重调节剂。虽然它通过减少晶体生长、晶体与肾细胞的粘附以及诱导晶体内化进入细胞来抑制CaOx结石的形成，但白藜芦醇会促进晶体聚集，而这是导致肾结石形成的机制之一。
