Yagishita Haruya, Go Yasuhiro, Okamoto Kazuki, Arimura Nariko, Ikegaya Yuji, Sasaki Takuya
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan.
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Front Neurosci. 2024 Apr 17;18:1360432. doi: 10.3389/fnins.2024.1360432. eCollection 2024.
Hippocampal pyramidal neurons exhibit diverse spike patterns and gene expression profiles. However, their relationships with single neurons are not fully understood. In this study, we designed an electrophysiology-based experimental procedure to identify gene expression profiles using RNA sequencing of single hippocampal pyramidal neurons whose spike patterns were recorded in living mice. This technique involves a sequence of experiments consisting of juxtacellular recording and labeling, brain slicing, cell collection, and transcriptome analysis. We demonstrated that the expression levels of a subset of genes in individual hippocampal pyramidal neurons were significantly correlated with their spike burstiness, submillisecond-level spike rise times or spike rates, directly measured by electrophysiological recordings. Because this methodological approach can be applied across a wide range of brain regions, it is expected to contribute to studies on various neuronal heterogeneities to understand how physiological spike patterns are associated with gene expression profiles.
海马体锥体细胞呈现出多样的放电模式和基因表达谱。然而，它们与单个神经元之间的关系尚未完全明晰。在本研究中，我们设计了一种基于电生理学的实验程序，通过对在活体小鼠中记录了放电模式的单个海马体锥体细胞进行RNA测序，来鉴定基因表达谱。该技术涉及一系列实验，包括旁细胞记录与标记、脑切片、细胞收集以及转录组分析。我们证明，单个海马体锥体细胞中一部分基因的表达水平与通过电生理记录直接测量的其放电爆发性、亚毫秒级放电上升时间或放电频率显著相关。由于这种方法学途径可应用于广泛的脑区，有望为各种神经元异质性的研究做出贡献，以了解生理放电模式如何与基因表达谱相关联。
