Ohya Y, Guth P H
Microcirc Endothelium Lymphatics. 1985 Jun;2(3):331-48.
The effect of prostaglandin E2 and histamine and their interaction on microvascular permeability in the rat mesentery was examined using fluorescent in vivo microscopy. Leak of a fluorescein-albumin conjugate from microvessels was determined 3 min after the topical application of prostaglandin E2 and/or histamine. The size of the leak (micron 2) was quantitated using a grid over the face of the videomonitor. To determine the role of endogenous prostaglandin, some rats were pretreated with 10 mg kg-1 indomethacin intravenously. All studies were performed coded. Dose-response studies performed with either prostaglandin E2 or histamine alone revealed that each agent increased microvascular permeability in a dose-related fashion. On a molar basis, prostaglandin E2 was more potent than histamine in this regard. When a low dose of prostaglandin E2 was administered together with a low or intermediate dose of histamine, the size of the resulting leak was significantly greater than the sum of the size of leak produced by each agent given separately. When rats were pretreated with 10 mg kg-1 indomethacin intravenously to inhibit prostaglandin synthesis, the histamine dose response was shifted to the right. From these experiments, we conclude that: In the rat mesentery, both topical prostaglandin E2 and histamine increase microvascular permeability to macromolecules in a dose-related fashion. Prostaglandin E2 is significantly more potent than histamine in this regard. Both exogenous and endogenous prostaglandin potentiate histamine's effect on microvascular permeability.
采用荧光活体显微镜检查前列腺素E2和组胺及其相互作用对大鼠肠系膜微血管通透性的影响。在局部应用前列腺素E2和/或组胺3分钟后，测定微血管中荧光素 - 白蛋白结合物的渗漏情况。渗漏大小（平方微米）通过视频监视器屏幕上的网格进行定量。为了确定内源性前列腺素的作用，一些大鼠静脉注射10mg/kg消炎痛进行预处理。所有研究均采用编码方式进行。单独使用前列腺素E2或组胺进行的剂量反应研究表明，每种药物均以剂量相关的方式增加微血管通透性。在摩尔基础上，前列腺素E2在这方面比组胺更有效。当低剂量的前列腺素E2与低剂量或中等剂量的组胺一起给药时，产生的渗漏大小明显大于单独给予每种药物所产生的渗漏大小之和。当大鼠静脉注射10mg/kg消炎痛以抑制前列腺素合成进行预处理时，组胺剂量反应向右移动。从这些实验中，我们得出以下结论：在大鼠肠系膜中，局部应用前列腺素E2和组胺均以剂量相关的方式增加大分子对微血管的通透性。在这方面，前列腺素E2比组胺明显更有效。外源性和内源性前列腺素均增强组胺对微血管通透性的作用。
