Tairov M M, Bersimbaev R I, Salganik R I
Biokhimiia. 1984 Jan;49(1):25-31.
It was discovered that prostaglandin E2 (PGE2), but not histamine, increased the incorporation of 3H-N-acetyl-D-glucosamine and 14C-amino acids into the acid-insoluble protein fraction of isolated, mainly mucoid cells of rat gastric mucosa. The cAMP at the dose of 1 mM enhanced, like the PGE2, the synthesis of gastric mucoids. Cycloheximide inhibited the basal incorporation of labelled N-acetyl-D-glucosamine and the amino acid mixture by 28 and 72%, respectively, and blocked completely the PGE2 effect on glycoproteins formation. It is suggested that the PGE2, unlike histamine, enhances the biosynthesis of glycoproteins in the mucoid cells of rat gastric mucosa. The cAMP is believed to be a messenger of the PGE2 effect.
研究发现，前列腺素E2（PGE2）而非组胺，可增加3H-N-乙酰-D-葡萄糖胺和14C-氨基酸掺入大鼠胃黏膜分离的主要为黏液细胞的酸不溶性蛋白质部分。1 mM剂量的环磷酸腺苷（cAMP）与PGE2一样，可增强胃黏液的合成。放线菌酮分别抑制标记的N-乙酰-D-葡萄糖胺和氨基酸混合物的基础掺入28%和72%，并完全阻断PGE2对糖蛋白形成的作用。提示PGE2与组胺不同，可增强大鼠胃黏膜黏液细胞中糖蛋白的生物合成。cAMP被认为是PGE2作用的信使。
