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潘塔纳尔湿地南部微生物组与淡水抗性组的比较基因组学研究

A comparative genomics study of the microbiome and freshwater resistome in Southern Pantanal.

作者信息

de Oliveira André R, de Toledo Rós Bárbara, Jardim Rodrigo, Kotowski Nelson, de Barros Adriana, Pereira Ricardo H G, Almeida Nalvo Franco, Dávila Alberto M R

机构信息

Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.

Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil.

出版信息

Front Genet. 2024 Apr 18;15:1352801. doi: 10.3389/fgene.2024.1352801. eCollection 2024.

Abstract

This study explores the resistome and bacterial diversity of two small lakes in the Southern Pantanal, one in Aquidauana sub-region, close to a farm, and one in Abobral sub-region, an environmentally preserved area. metagenomic sequencing data from water column samples collected near and far from the floating macrophyte were used. The Abobral small lake exhibited the highest diversity and abundance of antibiotic resistance genes (ARGs), antibiotic resistance classes (ARGCs), phylum, and genus. RPOB2 and its resistance class, multidrug resistance, were the most abundant ARG and ARGC, respectively. Pseudomonadota was the dominant phylum across all sites, and was the most abundant genus considering all sites.

摘要

本研究探讨了潘塔纳尔湿地南部两个小湖泊的耐药基因组和细菌多样性,一个位于阿基多阿纳次区域,靠近一个农场,另一个位于阿博布拉尔次区域,是一个环境保护区。使用了从漂浮大型植物远近采集的水柱样本的宏基因组测序数据。阿博布拉尔小湖的抗生素抗性基因(ARG)、抗生素抗性类别(ARGC)、门和属的多样性和丰度最高。RPOB2及其抗性类别多药抗性分别是最丰富的ARG和ARGC。假单胞菌门是所有位点的优势门,并且是所有位点中最丰富的属。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/965d/11063290/e1e70a35df25/fgene-15-1352801-g001.jpg

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