State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Nanjing Hydraulic Research Institute, 223# Guangzhou Road, Nanjing, 210029, China.
Key Laboratory of Surficial Geochemistry, Ministry of Education, School of Earth Sciences and Engineering, School of Environment, Nanjing University, Nanjing, China.
Environ Geochem Health. 2022 Aug;44(8):2531-2543. doi: 10.1007/s10653-021-01021-x. Epub 2021 Jul 22.
In recent decades, antibiotics and antibiotic resistance genes (ARGs) have been regarded as emerging pollutants. In Taihu Lake, as a typical representative of freshwater system in China, the ARGs occurrence and abundance was of great importance for ecological risk control and public health protection. In this research, high-throughput sequencing and metagenomics technique were used to investigate the seasonal ARGs profile in overlying water and sediment at typical area of Taihu Lake. Besides, taxonomy pattern of ARGs host bacteria and potential pathogens were identified. The results showed that 33 ARG subtypes and 11 ARG types were detected in research area, among which bacitracin, multidrug and sulfonamides resistance gene were with the highest abundance. The relative abundance of ARGs in overlying water and sediment ranged from 1.68 to 661.05 ppm and from 1.93 to 49.47 ppm, respectively. ARG host (18 bacteria genus) were identified and annotated, among which Clostridium botulinum, Pseudomonas aeruginosa and Klebsiella pneumonia were pathogenic bacteria. The pathogens were mostly detected at Xukou Bay in spring and fall, which might be caused by the inlet water from aquaculture area of Yangcheng Lake. Pseudomonas was the most abundant ARGs host (ant2ib, baca, bl2d_oxa2,mexb, mexf, mexw and oprn), which may facilitate the propagation of ARGs in freshwater system.
近几十年来，抗生素和抗生素耐药基因（ARGs）被认为是新兴的污染物。在中国典型的淡水系统太湖中，ARGs 的出现和丰度对生态风险控制和公共卫生保护具有重要意义。本研究采用高通量测序和宏基因组学技术，调查了太湖典型区域上覆水和底泥中季节性 ARGs 特征。此外，还确定了 ARGs 宿主细菌和潜在病原菌的分类模式。结果表明，研究区域共检测到 33 种 ARG 亚型和 11 种 ARG 类型，其中杆菌肽、多药和磺胺类耐药基因丰度最高。上覆水和底泥中 ARGs 的相对丰度分别为 1.68-661.05 ppm 和 1.93-49.47 ppm。鉴定并注释了 18 种 ARG 宿主（细菌属），其中肉毒梭菌、铜绿假单胞菌和肺炎克雷伯菌为病原菌。病原体主要在春季和秋季的胥口湾检测到，这可能是由于阳澄湖养殖区的进水所致。假单胞菌是最丰富的 ARGs 宿主（ant2ib、baca、bl2d_oxa2、mexb、mexf、mexw 和 oprn），这可能有利于淡水系统中 ARGs 的传播。
