Tulić Lidija, Tulić Ivan, Stojnić Jelena, Bila Jovan, Vuković Željka, Kotlica Boba
University of Belgrade, School of Medicine, Clinical Center of Serbia, Clinic for Gynecology and Obstetrics, Department of In Vitro Fertilization, Belgrade.
Clinical Center of Serbia, Clinic for Gynecology and Obstetrics, Belgrade.
J Med Biochem. 2024 Apr 23;43(2):219-225. doi: 10.5937/jomb0-46156.
This study aimed to examine if there is a connection between recombinant FSH dose and OS parameters in serum and the impact on IVF outcome.
This study consisted of 101 participants that went through IVF procedures. Parameter that were tested are SOD, SH groups and MDA. Serum samples were drawn before stimulation and on the last day of ovarian stimulation.
Two groups were formed according to the dose of gonadotropins (rFSH). In both groups there were no significant differences in live-birth rate and miscarriage. In both groups mean serum MDA and SH-groups were significantly higher after ovarian stimulation, but mean serum SOD was significantly lower when compared to values before stimulation. There were less patients without OS before stimulation.
Our results suggest that there is a difference in serum concentration in groups SOD, SH groups and MDA at the beginning and at the end ovarian stimulation. On the other hand, dose of rFSH is not related with change of parameters for oxidative stress, quality of oocytes, embryos, fertilization, pregnancies, and miscarriage rate. Patients without oxidative stress before the IVF procedure needed lower doses of gonadotropins during stimulation.
本研究旨在探讨重组促卵泡生成素（FSH）剂量与血清氧化应激参数之间是否存在关联，以及对体外受精（IVF）结局的影响。
本研究纳入了101例行IVF手术的参与者。检测的参数包括超氧化物歧化酶（SOD）、巯基（SH）基团和丙二醛（MDA）。在刺激前及卵巢刺激的最后一天采集血清样本。
根据促性腺激素（rFSH）剂量分为两组。两组的活产率和流产率均无显著差异。两组卵巢刺激后血清MDA和SH基团的平均值均显著升高，但与刺激前相比，血清SOD平均值显著降低。刺激前无氧化应激的患者较少。
我们的结果表明，卵巢刺激开始时和结束时，两组血清中SOD、SH基团和MDA的浓度存在差异。另一方面，rFSH剂量与氧化应激参数、卵母细胞质量、胚胎质量、受精、妊娠及流产率的变化无关。IVF手术前无氧化应激的患者在刺激期间需要较低剂量的促性腺激素。
