Vijayakumar Prasidhee, Dawson Paul A
Mater Research Institute, The University of Queensland, Woolloongabba QLD, Australia.
Essays Biochem. 2024 Dec 4;68(4):383-389. doi: 10.1042/EBC20230092.
Circulating sulfate needs to be maintained at sufficiently high levels for healthy growth and development. Animal studies have shown the adverse physiological consequences of low circulating sulfate level on the skeletal, neurological and reproductive systems. However, sulfate is not routinely measured in clinical investigations, despite the importance of sulfate being documented over the past several decades. Several methods have been developed for measuring serum and plasma sulfate level in animals and humans, including a range of barium sulfate precipitation techniques that have been a major focus of sulfate analytics since the 1960s. Evaluation of an ion chromatography method demonstrated its utility for investigation of sulfate levels in human health. More recently, liquid chromatography-tandem mass spectrometry has been used to show hyposulfatemia in a human case of mild skeletal dysplasia. This article provides an overview of analytical methods for measuring sulfate in serum and plasma, highlighting the strengths and limitations of each method.
循环中的硫酸盐需要维持在足够高的水平以实现健康的生长和发育。动物研究表明，循环中硫酸盐水平过低会对骨骼、神经和生殖系统产生不良生理影响。然而，尽管在过去几十年中已经证明了硫酸盐的重要性，但在临床研究中并不常规测量硫酸盐。已经开发了几种用于测量动物和人类血清及血浆中硫酸盐水平的方法，包括一系列硫酸钡沉淀技术，自20世纪60年代以来，这些技术一直是硫酸盐分析的主要重点。对一种离子色谱法的评估证明了其在研究人类健康中硫酸盐水平方面的实用性。最近，液相色谱-串联质谱法已被用于显示一例轻度骨骼发育不良人类病例中的低硫酸盐血症。本文概述了测量血清和血浆中硫酸盐的分析方法，突出了每种方法的优缺点。
