Lian Di, Tang Qiuyu, Wu Ling, Liao Xing
Respiratory Ward, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350005, Fujian, China.
Infection Diseases Ward, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350005, Fujian, China.
Medicine (Baltimore). 2024 May 3;103(18):e37757. doi: 10.1097/MD.0000000000037757.
To explore the effect of targeted second-generation sequencing technique to guide clinical diagnosis and medication on the therapeutic effect and prognosis of respiratory tract infection (RTI) in children. During January 2021 to June 2022, 320 children with RTI cured were selected in our hospital as the object of this retrospective study. The control group accepted empirical broad-spectrum antibacterial therapy and the observation group accepted targeted second-generation sequencing technique to guide diagnosis and medication. The therapeutic effect, improvement time of clinical symptom index, laboratory-related index, level of inflammatory factors, incidence of complications, and parents' treatment satisfaction were compared. The observation group was considerably more efficacious (91.25%) versus the controlled group (72.50%). The duration of enhancement of fever, nasal congestion, tonsillar congestion, and cough symptoms was shorter in the observation group (P < .05). Serum levels of iron, IgA, IgG as well as IgM were substantially elevated in the observation group. The levels of IL-4 and IL-10 were markedly reduced in the observation group after treatment. The prevalence of complications was considerably below that of the comparison group (21.25%) in the observation group (8.75%). Parental satisfaction with therapy was markedly higher in the observation group (92.50%) than in the control group (66.25%). The application of targeted second-generation sequencing technology to guide clinical diagnosis and drug use can elevate the RTIs efficacy and prognosis in childhood. Targeted second-generation sequencing can achieve precise treatment, reduce drug resistance of drug-resistant strains, and improve the efficacy. It has high promotion and application value.
探讨靶向二代测序技术指导临床诊断与用药对儿童呼吸道感染（RTI）治疗效果及预后的影响。选取2021年1月至2022年6月在我院治愈的320例RTI患儿作为本回顾性研究对象。对照组接受经验性广谱抗菌治疗，观察组接受靶向二代测序技术指导诊断与用药。比较两组的治疗效果、临床症状指标改善时间、实验室相关指标、炎症因子水平、并发症发生率及家长对治疗的满意度。观察组的有效率（91.25%）显著高于对照组（72.50%）。观察组发热、鼻塞、扁桃体充血及咳嗽症状的缓解时间更短（P < 0.05）。观察组血清铁、IgA、IgG及IgM水平显著升高。治疗后观察组IL-4和IL-10水平明显降低。观察组并发症发生率（8.75%）显著低于对照组（21.25%）。观察组家长对治疗的满意度（92.50%）明显高于对照组（66.25%）。应用靶向二代测序技术指导临床诊断和用药可提高儿童RTI的治疗效果及预后。靶向二代测序可实现精准治疗，降低耐药菌株的耐药性，提高疗效。具有较高的推广应用价值。
