MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Center for Healthcare Materials, Shaoxing Institute, Zhejiang University, Shaoxing 312099, China.
ACS Biomater Sci Eng. 2024 Jun 10;10(6):3946-3957. doi: 10.1021/acsbiomaterials.4c00363. Epub 2024 May 3.
Elevated levels of ROS, bacterial infection, inflammation, and improper regeneration are the factors that need to be addressed simultaneously for achieving effective wound healing without scar formation. This study focuses on the fabrication of electrospun ROS-responsive selenium-containing polyurethane nanofibers incorporating deferoxamine mesylate (Def), indomethacin (Indo), and gold nanorods (AuNRs) as proangiogenesis, anti-inflammatory, and antibacterial agents for synchronized delivery to a full-thickness wound . The structure of the fabricated nanofibers was analyzed by various techniques. Toxicity was checked by CCK-8 and hemolytic assays. The efficiency of wound healing was verified by a transwell assay and cell scratch assay. The wound healing efficiency of the nanofibers was assayed in full-thickness wounds in a rat model. The multifunctional nanofibers had a porous structure, enhanced antioxidation, antibacterial activity, and promoted wound healing. They eradicated TNF-α and IL-6, increased IL-10 expression, and revealed the angiogenic potential by increased expression of HIF-1α, VEGF, and CD31.
ROS 水平升高、细菌感染、炎症和不当再生是实现无瘢痕有效伤口愈合需要同时解决的因素。本研究专注于制备电纺 ROS 响应性含硒聚氨酯纳米纤维，将甲磺酸去铁胺（Def）、吲哚美辛（Indo）和金纳米棒（AuNRs）作为促血管生成、抗炎和抗菌剂，同步递送到全层伤口。通过各种技术分析了所制备纳米纤维的结构。通过 CCK-8 和溶血试验检查了毒性。通过 Transwell 测定和细胞划痕测定验证了伤口愈合效率。在大鼠全层伤口模型中测定了纳米纤维的伤口愈合效率。多功能纳米纤维具有多孔结构，增强了抗氧化、抗菌活性，并通过增加 HIF-1α、VEGF 和 CD31 的表达促进了伤口愈合。它们消除了 TNF-α 和 IL-6，增加了 IL-10 的表达，并显示出血管生成潜力。
