Chen Zhengrong, Mo Quansheng, Mo Dandan, Pei Xiaomin, Liang Anru, Cai Jinhong, Zhou Bo, Zheng Li, Li Hongmian, Yin Feiying, Zhao Jinmin
Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Key Laboratory of Regenerative Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
National & Regional United Engineering Lab of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Regen Biomater. 2024 Dec 14;12:rbae143. doi: 10.1093/rb/rbae143. eCollection 2025.
Injury caused by excess reactive oxygen species (ROS) may lead to susceptibility to bacterial infection and sustained inflammatory response, which are the major factors impeding diabetic wound healing. By utilizing optimal anti-inflammatory, antioxidant and antibacterial biomaterials for multifunctional wound dressings is critical in clinical applications. In this study, a novel electrospun PLGA/MoS@Pd nanofiber membrane was synthesized by encapsulating antioxidant and near-infrared (NIR) responsive MOS@Pd nanozymes in PLGA nanofibers to form a multifunctional dressing for diabetic wound repair. With excellent biocompatibility and hemostatic ability, this novel PLGA/MoS@Pd nanofiber membrane can effectively reduce oxidative stress damage and intracellular inflammatory factors expression in fibroblasts by scavenging ROS. Additionally, the PLGA/MoS@Pd nanofiber membrane exhibited favorable NIR-mediated photothermal antibacterial activity , with inhibition rates of 97.14% and 97.07% against () and (i), respectively. In a diabetic rat wound infection model, NIR-assisted PLGA/MoS@Pd nanofiber membrane effectively inhibited bacterial growth in the wound, reduced infection-induced inflammatory response, and promoted tissue epithelialization and collagen deposition, resulting in a wound healing rate of up to 98.5% on Day 14. This study highlighted the construction of a multifunctional nanofiber membrane platform and demonstrated its promising potential as a clinical dressing for diabetic wounds.
过量活性氧（ROS）造成的损伤可能导致易受细菌感染和持续的炎症反应，而这是阻碍糖尿病伤口愈合的主要因素。在临床应用中，利用具有最佳抗炎、抗氧化和抗菌性能的生物材料制作多功能伤口敷料至关重要。在本研究中，通过将抗氧化剂和近红外（NIR）响应性MOS@Pd纳米酶包裹在聚乳酸-羟基乙酸共聚物（PLGA）纳米纤维中，合成了一种新型的电纺PLGA/MoS@Pd纳米纤维膜，以形成用于糖尿病伤口修复的多功能敷料。这种新型PLGA/MoS@Pd纳米纤维膜具有优异的生物相容性和止血能力，能够通过清除ROS有效降低成纤维细胞中的氧化应激损伤和细胞内炎症因子表达。此外，PLGA/MoS@Pd纳米纤维膜表现出良好的近红外介导的光热抗菌活性，对（）和（i）的抑制率分别为97.14%和97.07%。在糖尿病大鼠伤口感染模型中，近红外辅助的PLGA/MoS@Pd纳米纤维膜有效抑制了伤口中的细菌生长，减少了感染引起的炎症反应，并促进了组织上皮化和胶原蛋白沉积，在第14天时伤口愈合率高达98.5%。本研究突出了多功能纳米纤维膜平台的构建，并证明了其作为糖尿病伤口临床敷料的广阔前景。
